basic methods in antibody production and characterization

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Basic Methods In Antibody Production And Characterization

Author : Gary C. Howard
ISBN : 142003653X
Genre : Medical
File Size : 76. 38 MB
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Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications. Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

Monoclonal Antibody Technology The Production And Characterization Of Rodent And Human Hybridomas

Author : A.M. Campbell
ISBN : 0080858821
Genre : Medical
File Size : 62. 31 MB
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This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Antibody Engineering

Author : Benny K. C. Lo
ISBN : 9781592596669
Genre : Medical
File Size : 77. 45 MB
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A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Use cutting-edge techniques for generating and optimizing recombinant antibodies Generate antibodies by humanization, molecular display technologies and transgenic mice Perform epitope mapping with mass spectrometry, bioinformatics, and array technologies Learn about novel applications in cancer, drug addiction, and proteomics Express recombinant antibodies in bacterial, yeast, insect, mammalian and plant systems.

Antibodies Applications And New Developments

Author : Eline P. Meulenberg
ISBN : 9781608052646
Genre : Medical
File Size : 81. 99 MB
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Antibodies Applications and New Developments is an overview of the current developments of techniques and methods relating to immunodiagnostics and immunoanalysis. This eBook also deals with specialties in the fields of drug, pesticide, antigen and food contaminant detection. The volume is useful for professional immunologists and biotechnologists interested in antibody research and development.

Immunological Methods

Author : Ivan Lefkovits
ISBN : 9781483265360
Genre : Medical
File Size : 25. 10 MB
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Immunological Methods, Volume IV provides information pertinent to the methods in immunological research. This book focuses on cells, clones, and cell lines, as well as on their components and secreted products. Organized into 21 chapters, this volume begins with an overview of hybridoma methodology as the most celebrated immunological method. This text then discusses cell fusion, hybridoma technology, and everything related to monoclonal antibodies. Other chapters consider another molecular biology method, which describes the procedure required for establishing a partitioned cDNA-library. This book provides as well a comprehensive analysis of mRNA populations in which every messenger species appears as a distinct element, and so provides accurate answers to questions concerning genetic complexity. The final chapter provides an example of how transgenic mice can be used to study the development of T cell repertoires. This book is a valuable resource for cell biologists, scientists, immunologists, and research workers.

Monoclonal Antibodies

Author : Maher Albitar
ISBN : 9781597453233
Genre : Medical
File Size : 49. 91 MB
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Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins. Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.

Antibodies In Cell Biology

Author :
ISBN : 0080859356
Genre : Medical
File Size : 28. 85 MB
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Antibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas. Practical applications and future emphases of antibodies, including: Light microscopic immunolocalization of antigens Gold particles in immunoelectron microscopy Special methods of fixation and permeabilization Microinjection of antibodies into living cells Antibodies to identify cDNA clones Antisense antibody strategies

Peptide Antibodies

Author : Gunnar Houen
ISBN : 1493929984
Genre : Medical
File Size : 89. 84 MB
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This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.

Making And Using Antibodies

Author : Gary C. Howard
ISBN : 9781439869109
Genre : Medical
File Size : 55. 68 MB
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Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science—both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications. Keeping pace with new developments in this area, all chapters in this new edition have been revised, updated, or expanded. Along with discussions of current applications, new material in the book includes chapters on western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and supply detailed descriptions of basic laboratory techniques. Each chapter begins with introductory material, allowing for a better understanding of each concept, and practical examples are included to help readers grasp the real-world scenarios in which antibodies play a part. From the eradication of smallpox to combating cancer, antibodies present an attractive solution to a range of biomedical problems. They are relatively easy to make and use, have great flexibility in applications, and are cost effective for most labs. This volume will assist biomedical researchers and students and pave the way for future discovery of new methods for making and using antibodies for a host of applications.

Microtubules In Vitro

Author : John J. Correia
ISBN : 9780124078888
Genre : Science
File Size : 61. 11 MB
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There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

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