bioconjugate techniques third edition

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Bioconjugate Techniques

Author : Greg T. Hermanson
ISBN : 9780123822406
Genre : Science
File Size : 33. 67 MB
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Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Bioconjugate Techniques

Author : Greg T. Hermanson
ISBN : 0080568726
Genre : Science
File Size : 45. 13 MB
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Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab *Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates *More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates *Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles Silane coupling agents Dendrimers and dendrons Chemoselective ligation Quantum dots Lanthanide chelates Cyanine dyes Discrete PEG compounds Buckyballs,fullerenes, and carbon nanotubes Mass tags and isotope tags Bioconjugation in the study of protein interactions

Bioconjugate Techniques

Author : Greg T. Hermanson
ISBN : 008057212X
Genre : Science
File Size : 28. 59 MB
Format : PDF, Mobi
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Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics Provides useful, detailed, easy-to-follow, step-by-step protocols Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates Cites over more than references keyed to concepts covered in the book Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications Suggests sources for all key reagents

Chemistry Of Bioconjugates

Author : Ravin Narain
ISBN : 9781118776377
Genre : Science
File Size : 31. 60 MB
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Explores bioconjugate properties and applications of polymers, dendrimers, lipids, nanoparticles, and nanotubes Bioconjugation has enabled breakthroughs across many areas of industry and biomedicine. With its emphasis on synthesis, properties and applications, this book enables readers to understand the connection between chemistry and the biological application of bioconjugated materials. Its detailed descriptions of methods make it possible for researchers to fabricate and take full advantage of bioconjugates for a broad range of applications. Moreover, the book sets the foundation for the development of new applications, including assays, imaging, biosensors, drug delivery, and diagnostics. Chemistry of Bioconjugates features contributions from an international team of leading experts and pioneers in the field. These contributions reflect the authors’ firsthand laboratory experience as well as a thorough review of the current literature. The book’s six sections examine: General methods of bioconjugation Polymer bioconjugates Organic nanoparticle-based bioconjugates Inorganic nanomaterial bioconjugates, including metals and metal oxides Cell-based, hydrogel/microgel, and glyco-bioconjugates Characterization, physico-(bio)chemical properties, and applications of bioconjugates This comprehensive exploration of bioconjugates includes discussions of polymers, dendrimers, lipids, nanoparticles, and nanotubes. References at the end of each chapter serve as a gateway to the most important original research findings and reviews in the field. By drawing together and analyzing all the latest chemical methods and research findings on the physico-chemical and biochemical properties of bioconjugates, Chemistry of Bioconjugates sheds new light on the significance and potential of bioconjugation. The book is recommended for organic and polymer chemists, biochemists, biomaterial scientists, carbohydrate chemists, biophysicists, bioengineers, and drug and gene delivery scientists.

Immobilized Affinity Ligand Techniques

Author : Greg T. Hermanson
ISBN : STANFORD:36105020876368
Genre : Science
File Size : 50. 33 MB
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This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Key Features * Matrix activation * Ligand immobilization * Binding and elution of target molecules * Enzyme catalysis on solid supports * Analytical affinity chromatography * Isolation/purification of antibodies * Preparation of antibody fragments * Immunoaffinity chromatography * Immobilization of nucleic acids * Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: * Matrices * Spacers * Methods of activation and coupling Background information and insights on: * Affinity interactions * The ease and power of affinity chromatography * Attaching molecules to insoluble supports * Matrices currently in use * Over 20 methods of activation * Spacers * Extensive References

Services Marketing Concepts Strategies Cases

Author : K. Douglas Hoffman
ISBN : 9781305855595
Genre : Business & Economics
File Size : 36. 45 MB
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Readers examine the use of services marketing as a competitive tool from a uniquely broad perspective with Hoffman/Bateson’s SERVICES MARKETING: CONCEPTS, STRATEGIES, AND CASES, 5E. Using a reader-friendly, streamlined structure, this book explores services marketing not only as an essential focus for service firms, but also as a competitive advantage for companies that market tangible products. A wealth of real examples feature a variety of businesses from industries both within and beyond the nine service economy supersectors: education and health services, financial activities, government, information, leisure and hospitality, professional and business services, transportation and utilities, wholesale and retail trade, and other services. Cutting-edge data addresses current issues, such as sustainability, technology, and the global market, giving readers valuable insights and important skills for success in business today. Important Notice: Media content referenced within the product description or the product text may not be available in the ebook version.

Bioanalytical Chemistry

Author : Susan R. Mikkelsen
ISBN : 9781118302545
Genre : Medical
File Size : 27. 21 MB
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A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry Provides an all in one approach for both beginners and experts, from a broad range of backgrounds, covering introductions, theory, advanced concepts and diverse applications for each method Each chapter progresses from basic concepts to applications involving real samples Includes three new chapters on Biomimetic Materials, Lab-on-Chip, and Analytical Methods Contains end-of-chapter problems and an appendix with selected answers

Solvents And Solvent Effects In Organic Chemistry

Author : Christian Reichardt
ISBN : 9783527324736
Genre : Science
File Size : 81. 4 MB
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Now in its 4th edition, this book remains the ultimate reference for all questions regarding solvents and solvent effects in organic chemistry. Retaining its proven concept, there is no other book which covers the subject in so much depth, the handbook is completely updated and contains 15% more content, including new chapters on "Solvents and Green chemistry", "Classification of Solvents by their Environmental Impact", and "Ionic Liquids". An essential part of every organic chemist's library.

Nanobiomaterials In Medical Imaging

Author : Alexandru Grumezescu
ISBN : 9780323417389
Genre : Medical
File Size : 63. 39 MB
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Nanobiomaterials in Medical Imaging presents the latest developments in medical exploratory approaches using nanotechnology. Leading researchers from around the world discuss recent progress and state-of-the-art techniques. The book covers synthesis and surface modification of multimodal imaging agents, popular examples of nanoparticles and their applications in different imaging techniques, and combinatorial therapy for the development of multifunctional nanocarriers. The advantages and potential of current techniques are also considered. This book will be of interest to postdoctoral researchers, professors and students engaged in the fields of materials science, biotechnology and applied chemistry. It will also be highly valuable to those working in industry, including pharmaceutics and biotechnology companies, medical researchers, biomedical engineers and advanced clinicians. A valuable resource for researchers, practitioners and students working in biomedical, biotechnological and engineering fields A detailed guide to recent scientific progress, along with the latest application methods Presents innovative opportunities and ideas for developing or improving technologies in nanomedicine and medical imaging

Basic Concepts Of Chemistry

Author : Leo J. Malone
ISBN : 9780471741541
Genre : Science
File Size : 82. 49 MB
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Engineers who need to have a better understanding of chemistry will benefit from this accessible book. It places a stronger emphasis on outcomes assessment, which is the driving force for many of the new features. Each section focuses on the development and assessment of one or two specific objectives. Within each section, a specific objective is included, an anticipatory set to orient the reader, content discussion from established authors, and guided practice problems for relevant objectives. These features are followed by a set of independent practice problems. The expanded Making it Real feature showcases topics of current interest relating to the subject at hand such as chemical forensics and more medical related topics. Numerous worked examples in the text now include Analysis and Synthesis sections, which allow engineers to explore concepts in greater depth, and discuss outside relevance.

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