biopunk solving biotech s biggest problems in kitchens and garages

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Biopunk

Author : Marcus Wohlsen
ISBN : 9781101476352
Genre : Science
File Size : 59. 27 MB
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Bill Gates recently told Wired that if he were a teenager today, he would be hacking biology. "If you want to change the world in some big way," he says, "that's where you should start-biological molecules." The most disruptive force on the planet resides in DNA. Biotech companies and academic researchers are just beginning to unlock the potential of piecing together life from scratch. Champions of synthetic biology believe that turning genetic code into Lego-like blocks to build never-before-seen organisms could solve the thorniest challenges in medicine, energy, and environmental protection. But as the hackers who cracked open the potential of the personal computer and the Internet proved, the most revolutionary discoveries often emerge from out-of-the-way places, forged by brilliant outsiders with few resources besides boundless energy and great ideas. In Biopunk, Marcus Wohlsen chronicles a growing community of DIY scientists working outside the walls of corporations and universities who are committed to democratizing DNA the way the Internet did information. The "biohacking" movement, now in its early, heady days, aims to unleash an outbreak of genetically modified innovation by making the tools and techniques of biotechnology accessible to everyone. Borrowing their idealism from the worlds of open-source software, artisinal food, Internet startups, and the Peace Corps, biopunks are devoted advocates for open-sourcing the basic code of life. They believe in the power of individuals with access to DNA to solve the world's biggest problems. You'll meet a new breed of hackers who aren't afraid to get their hands wet, from entrepreneurs who aim to bring DNA-based medical tools to the poorest of the poor to a curious tinkerer who believes a tub of yogurt and a jellyfish gene could protect the world's food supply. These biohackers include: -A duo who started a cancer drug company in their kitchen -A team who built an open-source DNA copy machine -A woman who developed a genetic test in her apartment for a deadly disease that had stricken her family Along with the potential of citizen science to bring about disruptive change, Wohlsen explores the risks of DIY bioterrorism, the possibility of genetic engineering experiments gone awry, and whether the ability to design life from scratch on a laptop might come sooner than we think.

Biopunk

Author : Marcus Wohlsen
ISBN : 1617230073
Genre : Science
File Size : 62. 46 MB
Format : PDF
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Wohlsen delivers a chronicle of DIY biotech scientists and their idealistic quest to democratize DNA like the Internet did information.

Biology Is Technology

Author : Robert H. Carlson
ISBN : 9780674053625
Genre : Science
File Size : 63. 39 MB
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In Biology Is Technology, author Robert Carlson offers a uniquely informed perspective on the endeavors that contribute to current progress in the science of biological systems and the technology used to manipulate them.

Life At The Speed Of Light

Author : J. Craig Venter
ISBN : 9780143125907
Genre : Science
File Size : 85. 59 MB
Format : PDF, Kindle
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The author of A Life Decoded explains how his team's achievement with sequencing the human genome has launched an important age of biological research, revealing a growing potential for enabling humans to adapt and evolve for long-term survival and environmental improvement.

Synthetic Biology

Author : Josefine Liljeruhm
ISBN : 9789814579568
Genre : Science
File Size : 60. 91 MB
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Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.” Read about the interview with the authors! Contents:Introduction:What is Synthetic Biology, Exactly?The iGEM OutbreakA Synthetic Biology Lab ManualGenes, Chromoproteins and Antisense RNAs:E. coli DNA: Chromosomes, Plasmids and Copy NumberCoupling of Transcription and Translation in BacteriaPromoter and Terminator for TranscriptionRibosome Binding Site (RBS)Codon BiasChromoproteinsSmall Regulatory RNAs (sRNAs)Lab Rooms and Equipment:The Physical Lab SpacesEquipmentSafety is Priority #1:FiresChemicalsBiological Safety and DisposalDangerous EquipmentLab Course Projects:Time and ResourcesProject Overview and Learning ObjectivesThe Lab NotebookLab Section 1. Preparation of Chemical Solutions and Agar PlatesLab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein GeneLab Section 3: Rational Engineering of Chromoprotein Expression LevelLab Section 4. Other ExperimentsThe “Dreaded” ExamProtocols:IntroductionProtocol 1. Preparation of Solutions and Agar PlatesProtocol 2. Overnight Cultures with Antibiotics, and Glycerol StocksProtocol 3. BioBrick™ 3A Assembly and Gel AnalysisProtocol 4. Agarose Gel ElectrophoresisProtocol 5. Preparation of Competent E. coli Cells Using CaCl2Protocol 6. Transformation of CaCl2-Competent E. coli CellsProtocol 7. Bacterial Re-Streaking TechniquesProtocol 8. Lysis of E. coli Cells with LysozymeProtocol 9. Polymerase Chain Reaction (PCR)Protocol 10. Inverse PCR MutagenesisProtocol 11. Colony PCRProtocol 12. Gibson AssemblyAdvanced Methods:Flow Cytometry and Cell SortingRecombination in Plasmids and the ChromosomeElectrocompetent CellsThe International Genetically Engineered Machine (iGEM) Competition:How to Start an iGEM TeamUppsala iGEM 2011 — Show Color with ColorUppsala iGEM 2012 — Resistance is FutileUppsala iGEM 2013 — Lactonutritious — It's DeliciousAppendices Readership: Students and researchers in biotechnology, cell/molecular biology and genetics. Keywords:Synthetic Biology;Genetic Engineering;DNA Cloning;Polymerase Chain Reaction;Molecular Biology;Laboratory;Biobrick;Chromoprotein;Manual;CourseReviews: It provides an introductory workflow for common techniques and would be of definite use to educators wanting to bring synthetic biology into the classroom or for motivated undergraduate students with the desire to start thir own iGEM team.” Synthetic Biology See Full Review

An Introduction To Genetic Engineering

Author : Desmond S. T. Nicholl
ISBN : 1139471783
Genre : Science
File Size : 56. 99 MB
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In this third edition of his popular undergraduate-level textbook, Des Nicholl recognises that a sound grasp of basic principles is vital in any introduction to genetic engineering. Therefore, the book retains its focus on the fundamental principles used in gene manipulation. It is divided into three sections: Part I provides an introduction to the relevant basic molecular biology; Part II, the methods used to manipulate genes; and Part III, applications of the technology. There is a new chapter devoted to the emerging importance of bioinformatics as a distinct discipline. Other additional features include text boxes, which highlight important aspects of topics discussed, and chapter summaries, which include aims and learning outcomes. These, along with key word listings, concept maps and a glossary, will enable students to tailor their study to suit their own learning styles and ultimately gain a firm grasp of a subject that students traditionally find difficult.

Genesis Machines

Author : Martyn Amos
ISBN : 9781782394914
Genre : Computers
File Size : 21. 32 MB
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Silicon chips are out. Today's scientists are using real, wet, squishy, living biology to build the next generation of computers. Cells, gels and DNA strands are the 'wetware' of the twenty-first century. Much smaller and more intelligent, these organic computers open up revolutionary possibilities. Tracing the history of computing and revealing a brave new world to come, Genesis Machines describes how this new technology will change the way we think not just about computers - but about life itself

Jack Kirby Pencils And Inks Artisan Edition

Author : Jack Kirby
ISBN : 1631407589
Genre :
File Size : 90. 42 MB
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Jack Kirby has been called the King of Comics, and rightfully so. For more than 40 years he was the most vital and groundbreaking artist in the medium. There have been dozens of books on Kirby over the years, including several oversized Artist's Editions showcasing his original art. This book will present Kirby's art in a new and unprecedented way, as side-by-side examples of his work, both in PENCIL and INK! Three Key first issues are showcased: The Demon #1, Kamandi #1 and Omac #1, along with a number of additional pieces presented. This is a perfect way to see Kirby art in its rawest form, from photocopies made from the original pencils as each page was completed, then next to it, the finished inks. We would almost call this a textbook of comic art except it's too much fun!

Atlas Of Crabs Of The Persian Gulf

Author : Reza Naderloo
ISBN : 9783319493749
Genre : Science
File Size : 74. 94 MB
Format : PDF
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This illustrated atlas describes 256 extant brachyuran crab species in the Persian Gulf and the Gulf of Oman. Identification keys are provided for 37 brachyuran families, 144 genera and 256 species on the basis of their main synapomorphies. Brief but precise descriptions highlighting the main characteristics are also provided for every family. The atlas displays features high-quality color photos, offering a hands-on guide and equipping readers to readily diagnose crab species in the region. Importantly, a line drawing of the first male gonopod, as well as its main diagnostic characteristics, are provided for all species. Further, every species is supplemented with synonymies that encompass the original descriptions, overall revision of the given taxa, monographs and all records from the northwestern Indian Ocean including the Persian Gulf and the Gulf of Oman. For each species, the book provides detailed local and global distribution maps, together with important ecological data including habitat preference. Further, it includes a general introduction to the brachyuran crabs with schematic drawings of their external morphology, as well as a comprehensive introduction to the Persian Gulf and the Gulf of Oman as marine ecoregions (geography, hydrology, biology, and environmental condition). The book offers an indispensable guide for all professionals, researchers, and students interested in brachyuran crabs around the globe and particularly in the Persian Gulf and the Gulf of Oman.

The Science Of Human Perfection

Author : Nathaniel Comfort
ISBN : 9780300169911
Genre : Medical
File Size : 33. 79 MB
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Almost daily we hear news stories, advertisements, and scientific reports promising that genetic medicine will make us live longer, enable doctors to identify and treat diseases before they harm us, and individualize our medical care. But surprisingly, a century ago eugenicists were making the same promises. This book traces the history of the promises of medical genetics and of the medical dimension of eugenics. While mindful of the benefits of genetic medicine, the book also considers social and ethical issues that cast troublesome shadows over these fields. Keeping his focus on America, Nathaniel Comfort introduces the community of scientists, physicians, and public health workers who have contributed to the development of medical genetics from the nineteenth century to today. He argues that medical genetics is closely related to eugenics, and indeed that the two cannot be fully understood separately. He also carefully examines how the desire to relieve suffering and to improve ourselves genetically, though noble, may be subverted. History makes clear that as patients and consumers we must take ownership of genetic medicine, using it intelligently, knowledgeably, and skeptically.

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