cell biological applications of confocal microscopy 70 methods in cell biology

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Cell Biological Applications Of Confocal Microscopy

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ISBN : 008049658X
Genre : Science
File Size : 36. 51 MB
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This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Biophysical Tools For Biologists

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ISBN : 9780080919782
Genre : Science
File Size : 40. 81 MB
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Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method

Ecological Biochemistry

Author : Gerd-Joachim Krauss
ISBN : 9783527316502
Genre : Science
File Size : 27. 59 MB
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The first stand-alone textbook for at least ten years on this increasingly hot topic in times of global climate change and sustainability in ecosystems. Ecological biochemistry refers to the interaction of organisms with their abiotic environment and other organisms by chemical means. Biotic and abiotic factors determine the biochemical flexibility of organisms, which otherwise easily adapt to environmental changes by altering their metabolism. Sessile plants, in particular, have evolved intricate biochemical response mechanisms to fit into a changing environment. This book covers the chemistry behind these interactions, bottom up from the atomic to the system's level. An introductory part explains the physico-chemical basis and biochemical roots of living cells, leading to secondary metabolites as crucial bridges between organisms and the respective ecosystem. The focus then shifts to the biochemical interactions of plants, fungi and bacteria within terrestrial and aquatic ecosystems with the aim of linking biochemical insights to ecological research, also in human-influenced habitats. A section is devoted to methodology, which allows network-based analyses of molecular processes underlying systems phenomena. A companion website offering an extended version of the introductory chapter on Basic Biochemical Roots is available at http://www.wiley.com/go/Krauss/Nies/EcologicalBiochemistry

Molecular Biology Of The Cell

Author : Bruce Alberts
ISBN : 9781317563747
Genre : Science
File Size : 42. 62 MB
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Techniques In Confocal Microscopy

Author : P. Michael Conn
ISBN : 0123846595
Genre : Science
File Size : 62. 31 MB
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As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. Documents many diverse uses for confocal microscopy in disciplines that broadly span biology The methods presented include shortcuts and conveniences not included in the initial publications Techniques are described in a context that allows comparisons to other related methodologies Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations

Handbook Of Biological Confocal Microscopy

Author : James Pawley
ISBN : 9781461571339
Genre : Medical
File Size : 30. 7 MB
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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Biochemistry And Molecular Biology Compendium

Author : Roger L. Lundblad
ISBN : 142004348X
Genre : Medical
File Size : 30. 73 MB
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While biomedical investigation has greatly advanced, investigators have lost touch with and inadvertently corrupted significant nomenclature at the foundation of their science. Nowadays, one has to be an insider to even understand the titles of journals, as modern biochemists have a tendency to invent new terms to describe old phenomena and apply acronyms in a haphazard way. In addition, while the use of kits now saves time, by taking shortcuts, many have lost touch with the principles that lie behind the processes they employ. Assembled by Roger Lundblad, the Biochemistry and Molecular Biology Compendium provides both academic and industrial researchers with an exceptionally accessible resource that offers a plethora of practical information not found in more database-oriented resources. A renowned scientist and author who bridges the old school of protein research and current proteomics, Dr. Lundblad is uniquely qualified to bring forth this handy resource. With great respect for the roots of the science, Dr. Lundblad provides a list of commonly used acronyms with definitions, as well as a glossary of terms and subjects used in biochemistry, molecular biology, biotechnology, proteomics, genomics, and systems biology. He also provides a chapter on those chemicals commonly employed in biochemistry and molecular biology, complete with properties and structure drawings, as well as a detailed accounting of protease inhibitors and protease inhibitor cocktails. A list of organic name reactions used in biochemistry is also included, as is a list of buffers with references to specific uses and unwanted side reactions. Until now, this information could only be garnered from older books and Internet searches convoluted by uncertain nomenclature. Biochemistry and Molecular Biology Compendium may not provide all the answers, but researchers will find it to be a valuable tool that will save them time, as well as provide essential links to the roots of their science.

Digital Image Analysis Of Microbes

Author : M. H. F. Wilkinson
ISBN : 0471974404
Genre : Science
File Size : 58. 2 MB
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Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.

Scanning Electron Microscopy For The Life Sciences

Author : Heide Schatten
ISBN : 9780521195997
Genre : Science
File Size : 29. 66 MB
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A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Xenopus Protocols

Author : X. Johné Liu
ISBN : 9781597450003
Genre : Science
File Size : 74. 98 MB
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This volume complements the larger body of literature on the subject by focusing on the versatility of frog oocytes and egg extracts in cell biology and signal transduction. All of the unique advantages of using frog oocytes as an experimental model are enthusiastically presented including pioneering work in the assembly of infectious virus particles via co-injection of genomic viral nucleic acids and capsid mRNAs. Xenopus Protocols: Cell Biology and Signal Transduction serves the scientific community by outlining the many possibilities this system has to offer and explores the possibility that Xenopus oocytes will serve prominently as a cell-based model to study functional genomics and proteomics in the post-genomic era.

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