computational approaches for studying enzyme mechanism part b methods in enzymology

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Computational Approaches For Studying Enzyme Mechanism

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ISBN : 9780128111086
Genre : Medical
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Computational Approaches for Studying Enzyme Mechanism, Part B is the first of two volumes in the Methods in Enzymology series that focuses on computational approaches for studying enzyme mechanism. The serial achieves the critically acclaimed gold standard of laboratory practices and remains one of the most highly respected publications in the molecular biosciences. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 550 volumes, the series remains a prominent and essential publication for researchers in all fields of the life sciences and biotechnology, including biochemistry, chemical biology, microbiology, synthetic biology, cancer research, genetics, and other fields of study. Focuses on computational approaches for studying enzyme mechanism Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in intermediate filament associated proteins, and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

Using Mass Spectrometry For Biochemical Studies On Enzymatic Domains From Polyketide Synthases

Author : Matthew Jenner
ISBN : 9783319327235
Genre : Science
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This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis. In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC. KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Complex Enzymes In Microbial Natural Product Biosynthesis Part B Polyketides Aminocoumarins And Carbohydrates

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ISBN : 0080923364
Genre : Science
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Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. * Gathers tried and tested methods and techniques from top players in the field. * Provides an extremely useful reference for the experienced research scientist. * Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates

Rna Ligand Interactions Part B Molecular Biology Methods

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ISBN : 9780080496757
Genre : Science
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RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods. RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Molecular Design And Modeling

Author : John Joseph Langone
ISBN : UVA:X002029280
Genre : Medical
File Size : 66. 62 MB
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Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein folding and ligand-acceptor interactions are included in Volumes 202 and 203, as are genetic and chemical methods for the production of functional molecules including antibodies and antigens, enzymes, receptors, nucleic acids and polysaccharides, and drugs.

Molecular Design And Modeling

Author : John Joseph Langone
ISBN : UVA:X002029280
Genre : Medical
File Size : 68. 16 MB
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Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein folding and ligand-acceptor interactions are included in Volumes 202 and 203, as are genetic and chemical methods for the production of functional molecules including antibodies and antigens, enzymes, receptors, nucleic acids and polysaccharides, and drugs.

Computational Approaches To Biochemical Reactivity

Author : Gábor Náray-Szabó
ISBN : UOM:39015040530456
Genre : Science
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Summarizes recent results in the field, presenting different approaches that can be useful in theoretical treatments of biological activities and in modeling and analyzing the energetics and kinetics of enzymatic reactions. Contains chapters on quantum mechanical models for reactions in solution, hybrid potentials for molecular systems in the condensed phase, modeling of protein transfer reactions in enzymes, and protein-ligand interactions. For graduate students and researchers working in molecular physics, structural biology, and structure-based molecular design. Annotation copyrighted by Book News, Inc., Portland, OR

Subject Guide To Books In Print

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ISBN : STANFORD:36105025888517
Genre : American literature
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Protein Structure

Author : Daniel Chasman
ISBN : 9780824748166
Genre : Medical
File Size : 43. 13 MB
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This text offers in-depth perspectives on every aspect of protein structure identification, assessment, characterization, and utilization, for a clear understanding of the diversity of protein shapes, variations in protein function, and structure-based drug design. The authors cover numerous high-throughput technologies as well as computational methods to study protein structures and residues. A valuable reference, this book reflects current trends in the effort to solve new structures arising from genome initiatives, details methods to detect and identify errors in the prediction of protein structural models, and outlines challenges in the conversion of routine processes into high-throughput platforms.

Nachrichten Aus Chemie Technik Und Laboratorium

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ISBN : CORNELL:31924056145752
Genre : Chemistry
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