enzymology primer for recombinant dna technology

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Enzymology Primer For Recombinant Dna Technology

Author : Hyone-Myong Eun
ISBN : 008053113X
Genre : Science
File Size : 72. 75 MB
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Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Enzymology Primer For Recombinant Dna Technology

Author : Enzymology Primer For Recombainant Dna Technology
ISBN : 8131201945
Genre :
File Size : 30. 93 MB
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Recombinant Dna Methodology Ii

Author : Ray Wu
ISBN : 9780323137744
Genre : Science
File Size : 62. 13 MB
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The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. * Methods for: * DNA isolation and cloning * Synthesizing complementary DNA (cDNA) * Cleaving and manipulating DNA * Selecting useful reporter genes * Constructing vectors for cloning genes * Constructing expression vectors * Site-directed mutagenesis and gene disruption * Identifying and mapping genes * Transforming animal and plant cells * Sequencing DNA * Amplifying and manipulating DNA and PCR * Detecting DNA - protein interaction

Molecular Biology

Author : Susan J. Karcher
ISBN : 0080536832
Genre : Science
File Size : 44. 13 MB
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This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)

Recombinant Dna Principles And Methodologies

Author : James Greene
ISBN : 0824799895
Genre : Science
File Size : 82. 30 MB
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This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Molecular Biotechnology

Author : Bernard R. Glick
ISBN : 1555819362
Genre : Biotechnology
File Size : 38. 97 MB
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Explains the many ways that recombinant microbes are harnessed in the service of humankind

Gene Cloning And Dna Analysis

Author : T. A. Brown
ISBN : 9781119072546
Genre : Science
File Size : 77. 70 MB
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Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis: In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes New material on the use of ChiP-seq to locate protein-binding sites Extended coverage of the strategies used to assemble genome sequences Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.

Guide To Yeast Genetics And Molecular Cell Biology

Author :
ISBN : 9780080496986
Genre : Science
File Size : 50. 95 MB
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This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.

Chemistry

Author : J. J. Lagowski
ISBN : 002865725X
Genre : Science
File Size : 82. 99 MB
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"Although written for the high school student, this concise, readable, and authoritative source presents any science learner with a clear introduction to chemistry. The 480 entries cover all aspects of chemistry and its practical applications. A running glossary explains words as they are encountered in the text, and most entries have accompanying diagrams and photographs."--"Reference that rocks," American Libraries, May 2005.

Molecular Biology Of The Cell

Author : Bruce Alberts
ISBN : 9781317563747
Genre : Science
File Size : 45. 79 MB
Format : PDF, Kindle
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

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