essentials in modern hplc separations

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Essentials In Modern Hplc Separations

Author : Serban C. Moldoveanu
ISBN : 9780123850133
Genre : Science
File Size : 83. 46 MB
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This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc. Gives a clear presentation of notions and conceptsDiscusses key parameters in HPLC separationIncludes modern developments in HPLCDescribes interrelation between various HPLC features (solvent pressure, separation, detection)Includes a large number of references.

Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : 9781118591512
Genre : Science
File Size : 37. 26 MB
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Introduction To Modern Liquid Chromatography

Author : Lloyd R. Snyder
ISBN : 9781118210390
Genre : Science
File Size : 35. 90 MB
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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 9781119293606
Genre : Science
File Size : 72. 22 MB
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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Separations Chemistry

Author : Fedor Macášek
ISBN : 9781514417300
Genre : Science
File Size : 47. 16 MB
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Separation of chemical species is a gate to final success of synthesis and preparation of compounds in pure and defined state. Variability of natural and artificial mixtures to be treated is enormous. Task of chemistry is to separate components of homogeneous mixtures (the gaseous and liquid solutions). The book concentrates on understanding the basic philosophies of both equilibrium and nonequilibrium chemical thermodynamics and engineering performance that lay in principle of separation technique such as distillation, crystallization, centrifugation, sorption, membrane separations, chromatography, and liquid-liquid extraction. Specific phenomena connected with photochemical separation, isotope composition, and radioactivity are discussed as well. The book is written for advanced students of chemistry having the knowledge of physical chemistry. Calculation examples are based on the international system of units. Unique list of over 1,300 full references covers scientific literature of the eighteenth to the twenty-first centuries.

Liquid Chromatography

Author : Salvatore Fanali
ISBN : 9780124158672
Genre : Science
File Size : 70. 93 MB
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A single source of authoritative information on all aspects of the practice of modern liquid chromatography suitable for advanced students and professionals working in a laboratory or managerial capacity Chapters written by authoritative and visionary experts in the field provide an overview and focused treatment of a single topic Comprehensive coverage of modern liquid chromatography from theory, to methods, to selected applications Thorough selected references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision making Extensive original tables and figures, placing recent research developments into a general context Worked examples, intuitive explanations, and clear figures reinforce learning

Liquid Chromatography

Author : Salvatore Fanali
ISBN : 9780128093443
Genre : Science
File Size : 53. 9 MB
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Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Hplc For Pharmaceutical Scientists

Author : Yuri V. Kazakevich
ISBN : 9780470087947
Genre : Science
File Size : 64. 47 MB
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HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

Practical High Performance Liquid Chromatography

Author : Veronika R. Meyer
ISBN : 9781118681343
Genre : Science
File Size : 39. 25 MB
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Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

Essentials Of Modern Hearing Aids

Author : Todd A. Ricketts
ISBN : 9781635500585
Genre : Medical
File Size : 36. 57 MB
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Essentials of Modern Hearing Aids: Selection, Fitting, and Verification is a comprehensive textbook, ideal for graduate-level amplification courses in audiology programs. It also is the ultimate go-to reference for anyone fitting and dispensing hearing aids. This is truly an "A to Z" textbook, with topics including audiologic prefitting testing, needs assessment and treatment planning, hearing aid selection, verification, orientation and counseling, post-fitting follow-up, and real-world validation. Moreover, a substantial portion of the book reviews the underlying up-to-date design and function of digital hearing aid components, circuitry and processing, the wide assortment of hearing aid features, and the many algorithms that make these features work successfully. The world-renowned authors have included numerous prefitting and post-fitting tests that can be conducted along with step-by-step protocols for their administration and scoring. They also review the selection of hearing aid styles and fitting arrangements, explain the process of obtaining an ear impression, and making critical decisions regarding earmolds and hearing aid plumbing. Essentials of Modern Hearing Aidsprovides guidance for selecting prescriptive fitting approaches and detailed protocols for the use of behavioral measures and real-ear speech mapping to both verify the fitting and assess special hearing aid features. Follow-up care and validation options also are reviewed, with detailed guidelines for using a wide range of outcome measures. Key Features Included throughout the text are short paragraphs identified as "Technical Tips," "Key Concepts," "Things to Remember," "Points to Ponder," and "Soapbox" for quick reference.The text covers both foundation and direct clinical application.The veteran authors have over 100 years of combined experience in day-to-day patient care and clinical research.The book is infused with humor throughout! Written in an accessible and easy-to-read style, this text includes not only reference information, but also tools supported by research and clinical experience. The information is presented in a way that is both accessible to clinical students with little experience in the field and with enough depth for even the serious hearing aid researcher. The text is also appropriate as a handy companion for busy clinicians - a friendly resource where they can quickly find critical information needed for the next patient. *Disclaimer: Please note that ancillary content (such as documents, audio, and video, etc.) may not be included as published in the original print version of this book.

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