fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real time analysis biological techniques series

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Fluorescent And Luminescent Probes For Biological Activity

Author : W. T. Mason
ISBN : 9780080531779
Genre : Science
File Size : 79. 34 MB
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The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy

Measurement And Manipulation Of Intracellular Ions

Author :
ISBN : 9780080536460
Genre : Science
File Size : 37. 94 MB
Format : PDF
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This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures. Key Features * A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies * Includes overviews of * Optical probes and reagents * Fabrication and use of ion-selective microelectrodes * Use of NMR spectroscopy * Ionophores

Analytical Biotechnology

Author : Thomas G.M. Schalkhammer
ISBN : 9783034881012
Genre : Science
File Size : 71. 15 MB
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Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Imaging Living Cells

Author : Rosario Rizzuto
ISBN : 9783642600036
Genre : Science
File Size : 33. 73 MB
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Intended for bench-top use, this lab manual is suitable for both scientists and graduate students, since it combines an update on the most advanced imaging procedures with detailed protocols. Examples, carefully selected from the wide repertoire of cell pyhsiology, cover such different functional aspects as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Handbook Of Fluorescent Probes And Research Products

Author : Richard P. Haugland
ISBN : PSU:000055798648
Genre : Science
File Size : 62. 3 MB
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Luminescent Semiconductor Nanocrystals Intermittent Behavior And Use As Fluorescent Biological Probes

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ISBN : UCAL:C3409164
Genre :
File Size : 38. 14 MB
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Membrane Transport In Plants Fungi

Author : Michael Robert Blatt
ISBN : UVA:X002562949
Genre : Medical
File Size : 83. 29 MB
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Confocal Laser Scanning Microscopy

Author : Colin Sheppard
ISBN : 0387915141
Genre : Science
File Size : 37. 19 MB
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Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications. Paper. DLC: Confocal microscopy.

Methods Of Biochemical Analysis

Author : David Glick
ISBN : UVA:X002539102
Genre : Biochemistry
File Size : 30. 36 MB
Format : PDF
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Biosupplynet Source Book

Author :
ISBN : UOM:39015048668159
Genre : Scientific apparatus and instruments
File Size : 40. 7 MB
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