fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real time analysis biological techniques series

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Fluorescent And Luminescent Probes For Biological Activity

Author : W. T. Mason
ISBN : 9780080531779
Genre : Science
File Size : 44. 72 MB
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The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy

Measurement And Manipulation Of Intracellular Ions

Author :
ISBN : 9780080536460
Genre : Science
File Size : 50. 36 MB
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This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures. Key Features * A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies * Includes overviews of * Optical probes and reagents * Fabrication and use of ion-selective microelectrodes * Use of NMR spectroscopy * Ionophores

Analytical Biotechnology

Author : Thomas G.M. Schalkhammer
ISBN : 9783034881012
Genre : Science
File Size : 70. 57 MB
Format : PDF
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Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Imaging Living Cells

Author : Rosario Rizzuto
ISBN : 9783642600036
Genre : Science
File Size : 60. 33 MB
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Intended for bench-top use, this lab manual is suitable for both scientists and graduate students, since it combines an update on the most advanced imaging procedures with detailed protocols. Examples, carefully selected from the wide repertoire of cell pyhsiology, cover such different functional aspects as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Handbook Of Fluorescent Probes And Research Products

Author : Richard P. Haugland
ISBN : PSU:000055798648
Genre : Science
File Size : 60. 94 MB
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Luminescent Semiconductor Nanocrystals Intermittent Behavior And Use As Fluorescent Biological Probes

Author :
ISBN : UCAL:C3409164
Genre :
File Size : 32. 22 MB
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Cell Biology

Author : Julio E. Celis
ISBN : 0121647285
Genre : Science
File Size : 46. 44 MB
Format : PDF, Kindle
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This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together new and revised chapters. Each chapter is concisely written and beautifully illustrated, making the attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors. Key Features * Contains more than 40% new material * Provides cell biologists and other life scientists with the most up-to-date instructions for basic and advanced cell biological techniques, including those at the interface between cell and molecular biology * Features uniform style and editing and includes contributions from world-renowned authorities in their respective fields * Contains information appropriate for a large, diverse, and constantly growing international audience of cell, developmental, and molecular biologists, plus others who need these methods in their laboratory research * Includes color plates throughout the set for easy reference * Designed as the essential lab guide and research reference for the field

Confocal Laser Scanning Microscopy

Author : Colin Sheppard
ISBN : 0387915141
Genre : Science
File Size : 41. 53 MB
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Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications. Paper. DLC: Confocal microscopy.

Membrane Transport In Plants Fungi

Author : Michael Robert Blatt
ISBN : UVA:X002562949
Genre : Medical
File Size : 22. 24 MB
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Encyclopedia Of Cell Technology

Author : Raymond Spier
ISBN : 047116643X
Genre : Science
File Size : 69. 57 MB
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