fundamentals of light microscopy and electronic imaging

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Fundamentals Of Light Microscopy And Electronic Imaging

Author : Douglas B. Murphy
ISBN : 9780471234296
Genre : Science
File Size : 33. 83 MB
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Over the last decade, advances in science and technology haveprofoundly changed the face of light microscopy. Researchscientists need to learn new skills in order to use a modernresearch microscope–skills such as how to align microscopeoptics and perform image processing. Fundamentals of LightMicroscopy and Electronic Imaging explores the basics ofmicroscope design and use. The comprehensive material discusses theoptical principles involved in diffraction and image formation inthe light microscope, the basic modes of light microscopy, thecomponents of modern electronic imaging systems, and the imageprocessing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals ofLight Microscopy and Electronic Imaging reviews such topicsas: Illuminators, filters, and isolation of specificwavelengths Phase contrast and differential interference contrast Properties of polarized light and polarization microscopy Fluorescence and confocal laser scanning microscopy Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercisesalong with a discussion of the relevant material. In addition, athorough glossary assists with complex terminology and an appendixcontains lists of materials, procedures for specimen preparation,and answers to questions. An essential resource for both, experienced and novicemicroscopists.

Bioimaging Current Concepts In Light Electron Microscopy

Author : Douglas E Chandler
ISBN : 9780763738747
Genre : Medical
File Size : 47. 93 MB
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The development of microscopy revolutionized the world of cell and molecular biology as we once knew it and will continue to play an important role in future discoveries. Bioimaging: Current Concepts in Light and Electron Microscopy is the optimal text for any undergraduate or graduate bioimaging course, and will serve as an important reference tool for the research scientist. This unique text covers, in great depth, both light and electron microscopy, as well as other structure and imaging techniques like x-ray crystallography and atomic force microscopy. Written in a user-friendly style and covering a broad range of topics, Bioimaging describes the state-of-the-art technologies that have powered the field to the forefront of cellular and molecular biological research. Important Notice: The digital edition of this book is missing some of the images or content found in the physical edition.

Confocal Microscopy For Biologists

Author : Alan R. Hibbs
ISBN : 9780306485657
Genre : Science
File Size : 49. 91 MB
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There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.

Understanding Light Microscopy

Author : Jeremy Sanderson
ISBN : 9780470973752
Genre : Science
File Size : 69. 17 MB
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Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Wilhelm Reich Biologist

Author : James E. Strick
ISBN : 9780674286887
Genre : Science
File Size : 63. 69 MB
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Wilhelm Reich’s experiments in the 1930s with cutting-edge light microscopy and time-lapse micro-cinematography were considered discredited, but not because of shoddy lab technique, as has been claimed. Scientific opposition to Reich’s experiments, James Strick argues, grew out of resistance to his unorthodox sexual theories and Marxist leanings.

Cell Biology E Book

Author : Thomas D. Pollard
ISBN : 9781437700633
Genre : Medical
File Size : 45. 38 MB
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A masterful introduction to the cell biology that you need to know! This critically acclaimed textbook offers you a modern and unique approach to the study of cell biology. It emphasizes that cellular structure, function, and dysfunction ultimately result from specific macromolecular interactions. You'll progress from an explanation of the "hardware" of molecules and cells to an understanding of how these structures function in the organism in both healthy and diseased states. The exquisite art program helps you to better visualize molecular structures. Covers essential concepts in a more efficient, reader-friendly manner than most other texts on this subject. Makes cell biology easier to understand by demonstrating how cellular structure, function, and dysfunction result from specific macromole¬cular interactions. Progresses logically from an explanation of the "hardware" of molecules and cells to an understanding of how these structures function in the organism in both healthy and diseased states. Helps you to visualize molecular structures and functions with over 1500 remarkable full-color illustrations that present physical structures to scale. Explains how molecular and cellular structures evolved in different organisms. Shows how molecular changes lead to the development of diseases through numerous Clinical Examples throughout. Includes STUDENT CONSULT access at no additional charge, enabling you to consult the textbook online, anywhere you go · perform quick searches · add your own notes and bookmarks · follow Integration Links to related bonus content from other STUDENT CONSULT titles—to help you see the connections between diverse disciplines · test your knowledge with multiple-choice review questions · and more! New keystone chapter on the origin and evolution of life on earth probably the best explanation of evolution for cell biologists available! Spectacular new artwork by gifted artist Graham Johnson of the Scripps Research Institute in San Diego. 200 new and 500 revised figures bring his keen insight to Cell Biology illustration and further aid the reader’s understanding. New chapters and sections on the most dynamic areas of cell biology - Organelles and membrane traffic by Jennifer Lippincott-Schwartz; RNA processing (including RNAi) by David Tollervey., updates on stem cells and DNA Repair. ,More readable than ever. Improved organization and an accessible new design increase the focus on understanding concepts and mechanisms. New guide to figures featuring specific organisms and specialized cells paired with a list of all of the figures showing these organisms. Permits easy review of cellular and molecular mechanisms. New glossary with one-stop definitions of over 1000 of the most important terms in cell biology.

Handbook Of Fluorescent Probes And Research Products

Author : Richard P. Haugland
ISBN : PSU:000055798648
Genre : Science
File Size : 84. 96 MB
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The World Of The Cell

Author : Wayne M. Becker
ISBN : STANFORD:36105114190213
Genre : Science
File Size : 57. 44 MB
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Accompanying CD-ROM contains more than 80 animations and interactive activities, numerous microscopy video sequences that show cellular processes in action, a searchable glossary, flashcards, automatically graded practice quizzes, annotated web links, key terms, a gradebook, and access to Research Navigator.

Materials Characterization

Author : Yang Leng
ISBN : 0470822988
Genre : Technology & Engineering
File Size : 55. 26 MB
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This book covers state-of-the-art techniques commonly used in modern materials characterization. Two important aspects of characterization, materials structures and chemical analysis, are included. Widely used techniques, such as metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are described. In addition, the book introduces advanced techniques, including scanning probe microscopy. The second half of the book accordingly presents techniques such as X-ray energy dispersive spectroscopy (commonly equipped in the scanning electron microscope), fluorescence X-ray spectroscopy, and popular surface analysis techniques (XPS and SIMS). Finally, vibrational spectroscopy (FTIR and Raman) and thermal analysis are also covered.

Digital Microscopy

Author : Greenfield Sluder
ISBN : 0080544347
Genre : Science
File Size : 83. 79 MB
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The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

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