gnotobiotic mouse technology an illustrated guide

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Gnotobiotic Mouse Technology

Author : Chriss J. Vowles
ISBN : 9781498736336
Genre : Medical
File Size : 43. 95 MB
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The popularity of germ-free animal models, particularly mice, for investigation of human physiology and disease has recently exploded. Gnotobiotic Mouse Technology: An Illustrated Guide provides the first manual for the maintenance, husbandry, and experimental manipulation of germ-free and gnotobiotic mice. It includes information on all aspects of establishing and operating a germ-free mouse research facility, from basic principles and equipment to detailed instructions for assembling and maintaining isolators, sterilizing supplies, handling animals, and monitoring sterility. The book breaks down techniques and procedures into modules, each of which contains a step-by-step practical and visual guide to a set of related procedures. Each description consists of a materials list, introduction, and general overview, followed by a thoroughly illustrated walkthrough of the steps for each procedure. The descriptions end with troubleshooting tips. Technical chapters are heavily illustrated and include notations of potential pitfalls and alternatives. Other chapters discuss management procedures and practices such as the hiring and training of personnel, setting fee schedules, and record keeping. This book is a landmark resource for establishing and maintaining a facility for germ-free mouse research. Useful to both technicians and investigators, it presents every step necessary to establish a successful facility. It also gives direction in expanding multi-user facilities and applying new technologies to your current practices.

Germfree And Gnotobiotic Animal Models

Author : Bernard S. Wostmann
ISBN : 084934008X
Genre : Medical
File Size : 75. 78 MB
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The germfree animal is reared in the laboratory to be bacteria free; its counterpart, the gnotobiotic animal, is exposed to select microorganisms. The need for such an animal model for use in biomedical studies was first expressed by Pasteur in the late 1800s. Subsequent development of germfree and gnotobiotic animals led to an explosion of studies on the effects of microflora and its components on the physiology and metabolism of the host. Germfree and Gnotobiotic Animal Models brings together the most notable points of early and recent studies and gives reference to the most pertinent literature.

Gis And The Social Sciences

Author : Dimitris Ballas
ISBN : 9781317638827
Genre : Science
File Size : 30. 67 MB
Format : PDF, Docs
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GIS and the Social Sciences offers a uniquely social science approach on the theory and application of GIS with a range of modern examples. It explores how human geography can engage with a variety of important policy issues through linking together GIS and spatial analysis, and demonstrates the importance of applied GIS and spatial analysis for solving real-world problems in both the public and private sector. The book introduces basic theoretical material from a social science perspective and discusses how data are handled in GIS, what the standard commands within GIS packages are, and what they can offer in terms of spatial analysis. It covers the range of applications for which GIS has been primarily used in the social sciences, offering a global perspective of examples at a range of spatial scales. The book explores the use of GIS in crime, health, education, retail location, urban planning, transport, geodemographics, emergency planning and poverty/income inequalities. It is supplemented with practical activities and datasets that are linked to the content of each chapter and provided on an eResource page. The examples are written using ArcMap to show how the user can access data and put the theory in the textbook to applied use using proprietary GIS software. This book serves as a useful guide to a social science approach to GIS techniques and applications. It provides a range of modern applications of GIS with associated practicals to work through, and demonstrates how researcher and policy makers alike can use GIS to plan services more effectively. It will prove to be of great interest to geographers, as well as the broader social sciences, such as sociology, crime science, health, business and marketing.

Molecular Biology

Author : Jordanka Zlatanova
ISBN : 0815345046
Genre :
File Size : 62. 91 MB
Format : PDF
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Molecular Biology: Structure and Dynamics of Genomes and Proteomes illustrates the essential principles behind the transmission and expression of genetic information at the level of DNA, RNA, and proteins. This textbook emphasizes the experimental basis of discovery and the most recent advances in the field while presenting a structural, mechanistic understanding of molecular biology that is rigorous, yet concise. The text is written for advanced undergraduate or graduate-level courses in molecular biology.

A Field Manual Of Camel Diseases

Author : Paul Mundy
ISBN : 185339503X
Genre : Medical
File Size : 20. 61 MB
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The manual details major camel diseases and conditions with the disease signs, its causes, and simple prevention and treatment methods. Both scientific and tried and tested traditional treatments are presented, thus enabling the veterinarian or livestock practitioner to make the most appropriate choice in the prevailing circumstances.

Manual Of Environmental Microbiology Fourth Edition

Author : Cindy H. Nakatsu
ISBN : 1555816029
Genre :
File Size : 75. 24 MB
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Bacterial Pathogenesis

Author : Virginia L. Clark
ISBN : 0121821374
Genre : Science
File Size : 32. 98 MB
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This volume of Methods in Enzymology contains contributions covering the wide spectrum of interactions between bacterial pathogens and their eukaryotic hosts. It complements Volume 235, in which methods for the isolation and identification of bacterial pathogens and associated virulence determinants are described. Since the study of bacterial pathogenesis borrows from a great variety of technologies and disciplines, we aim to provide the reader with a representative sample of the most advanced pathogenesis research.

Behaviour Problems In Small Animals

Author : Jon Bowen (BVetMed.)
ISBN : 9780702027673
Genre : Medical
File Size : 79. 24 MB
Format : PDF, Kindle
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This compact, easy-access guide to behavioral problems encountered in veterinary practice offers immediate and helpful advice for today's veterinary team. Instructor resources are available; please contact your Elsevier sales representative for details. Coverage offers immediate advice to the veterinary team who are often presented with behavioral problems in their practice. Easy-to-read format includes introductions, medical differentials, underlying causes, diagnosis, action boxes, and summaries. Content refers the reader to other chapters throughout the book, so that information can be found quickly and easily. Helpful handouts, which can be photocopied, offer yet another way to further your learning experience.

The Laboratory Mouse Second Edition

Author : Peggy J. Danneman
ISBN : 9781439854211
Genre : Medical
File Size : 70. 72 MB
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Mice have long been recognized as a valuable tool for investigating the genetic and physiological bases of human diseases such as diabetes, infectious disease, cancer, heart disease, and a wide array of neurological disorders. With the advent of transgenic and other genetic engineering technologies, the versatility and usefulness of the mouse as a model in biomedical research has soared. As a result, mouse colonies everywhere are expanding, and scientists who previously focused on other models are turning their attention to the mouse. Revised to reflect advances since the first edition, The Laboratory Mouse, Second Edition continues to be the most accessible reference on the biology and care of the laboratory mouse. This guide presents basic information and common procedures in detail to provide a quick reference source for investigators, technicians, and caretakers in the humane care and use of the mouse in the laboratory setting. Expanded, updated, and now in color, this new edition includes coverage of the biological features, husbandry, management, veterinary care, experimental methodology, and resources applying specifically to the mouse.

The Laboratory Rat Second Edition

Author : Patrick Sharp
ISBN : 9781439829868
Genre : Medical
File Size : 54. 28 MB
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Rats have long been recognized as a valuable biomedical research model, notably in the investigation of aging, toxicology, addiction, and common human diseases such as diabetes and hypertension. In many instances, individuals conducting such research studies are charged with important responsibilities, including animal facility management, animal husbandry, veterinary care, regulatory compliance, and various experimental methodologies. With the advent of genetic manipulations and biomedical research technological advances such as bioimaging, the versatility and usefulness of the rat as an animal model has soared. The Laboratory Rat, Second Edition captures the multiple advances in this important animal model’s husbandry, veterinary care, and experimental methodology. This edition features augmented, expanded, and novel information on biology, anesthesia, analgesia, and experimental techniques benefiting personnel working with rats—from the animal care staff to the researcher and everyone in between. The book is also extremely useful to institutional animal care and use program supporters and elements, including animal care and use committees, institutional officials, occupational health and safety professionals, veterinary technicians, and veterinarians.

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