handbook of isoelectric focusing and proteomics volume 7 separation science and technology

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Handbook Of Isoelectric Focusing And Proteomics

Author : David Garfin
ISBN : 9780080533629
Genre : Science
File Size : 64. 55 MB
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Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Handbook Of Bioseparations

Author : Satinder Ahuja
ISBN : 0080507794
Genre : Technology & Engineering
File Size : 44. 3 MB
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It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments

Low Abundance Proteome Discovery

Author : Egisto Boschetti
ISBN : 9780124045965
Genre : Science
File Size : 73. 67 MB
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Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics. Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress Includes detailed protocols that address real-life needs in laboratory practice Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins

The Proteomics Protocols Handbook

Author : John M. Walker
ISBN : 9781592598908
Genre : Science
File Size : 36. 40 MB
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Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Handbook Of Capillary And Microchip Electrophoresis And Associated Microtechniques Third Edition

Author : James P. Landers
ISBN : 1420004956
Genre : Medical
File Size : 51. 42 MB
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Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.

Elektrophorese Praktikum

Author : Reiner Westermeier
ISBN : 352728172X
Genre : Science
File Size : 71. 88 MB
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Die Elektrophorese ist eine bedeutende Methode der Instrumentellen Analyse und vor allem aus den Laboratorien der Biochemiker nicht mehr wegzudenken. Dieses Buch bietet eine umfassende Methodenübersicht für alle, die die verschiedenen Varianten der modernen Elektrophorese kennenlernen oder die ihre Kenntnisse ausbauen wollen. Der Autor erläutert zunächst die physikalisch-chemischen Zusammenhänge und gibt dann zahlreiche praktische, an Anwendungsbeispielen orientierte Hinweise. Gründliche Arbeitsanleitungen und ausführliche Problemlösungen geben dem Praktiker eine wertvolle Hilfe für seine tägliche Arbeit. Reiner Westermeier ist Mitarbeiter am Institut für Lebensmitteltechnologie der TU München und in der internationalen Firmengruppe Pharmacia LKB tätig. Zahlreiche Veröffentlichungen und eine umfangreiche Vortrags- und Seminartätigkeit haben den promovierten Ingenieur zu einem bekannten und vielgefragten Fachmann auf dem Gebiet der Elektrophorese gemacht.

American Biotechnology Laboratory

Author :
ISBN : STANFORD:36105123660420
Genre : Bioengineering
File Size : 59. 84 MB
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Henry S Clinical Diagnosis And Management By Laboratory Methods E Book

Author : Richard A. McPherson
ISBN : 9781455726844
Genre : Medical
File Size : 27. 76 MB
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Recognized as the definitive book in laboratory medicine since 1908, Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of results. Revisions throughout keep you current on the latest topics in the field, such as biochemical markers of bone metabolism, clinical enzymology, pharmacogenomics, and more! A user-friendly full-color layout puts all the latest, most essential knowledge at your fingertips. Update your understanding of the scientific foundation and clinical application of today's complete range of laboratory tests. Get optimal test results with guidance on error detection, correction, and prevention as well as cost-effective test selection. Reference the information you need quickly and easily thanks to a full-color layout, many new color illustrations and visual aids, and an organization by organ system. Master all the latest approaches in clinical laboratory medicine with new and updated coverage of: the chemical basis for analyte assays and common interferences; lipids and dyslipoproteinemia; markers in the blood for cardiac injury evaluation and related stroke disorders; coagulation testing for antiplatelet drugs such as aspirin and clopidogrel; biochemical markers of bone metabolism; clinical enzymology; hematology and transfusion medicine; medical microbiology; body fluid analysis; and many other rapidly evolving frontiers in the field. Effectively monitor the pace of drug clearing in patients undergoing pharmacogenomic treatments with a new chapter on this groundbreaking new area. Apply the latest best practices in clinical laboratory management with special chapters on organization, work flow, quality control, interpretation of results, informatics, financial management, and establishing a molecular diagnostics laboratory. Confidently prepare for the upcoming recertification exams for clinical pathologists set to begin in 2016.

Gentechnologie F R Einsteiger

Author : Terence A. Brown
ISBN : 3827413028
Genre :
File Size : 47. 17 MB
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T. A. Browns "Gentechnologie fA1/4r Einsteiger" hat sich in mehreren Auflagen international als StandardeinfA1/4hrung in die Genklonierung und DNA-Analyse etabliert. Das Grundlagenlehrbuch setzt nur wenig Kenntnisse in Molekularbiologie und Genetik voraus und erlAutert in klarer Sprache sowohl die fundamentalen Prinzipien der wichtigsten gentechnologischen Methoden als auch die immer breiter werdende Palette ihrer Anwendungen. Die groAe Bedeutung der Gentechnologie fA1/4r die Grundlagenforschung und fA1/4r neue Entwicklungen in Biotechnologie und Medizin wird klar herausgearbeitet. Aoeber 250 zweifarbige Illustrationen erleichtern das VerstAndnis des Stoffes und machen die Konzepte und Arbeitstechniken der Gentechnologen anschaulich. Die neue Auflage ist vollstAndig A1/4berarbeitet und enthAlt zwei neue Kapitel. Eines prAsentiert die Methoden, mit denen man ganze Genome sequenziert, und beschreibt, was man aus den gewonnenen Sequenzinformationen ablesen kann. Das zweite widmet sich den Anwendungen der Gentechnologie in der Gerichtsmedizin - ein spannendes und aufschlussreiches Beispiel des Einsatzpotenzials der DNA-Analyse. Der groAen Bedeutung der Polymerasekettenreaktion (PCR) entsprechend wird diese Technik jetzt in einem frA1/4hen Abschnitt des Buches behandelt und durchgehend stArker berA1/4cksichtigt.

Biotechnologie In Der Lebensmittelindustrie

Author : M.P. Tombs
ISBN : 9783642786495
Genre : Science
File Size : 54. 68 MB
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