handbook of single molecule fluorescence spectroscopy

Download Book Handbook Of Single Molecule Fluorescence Spectroscopy in PDF format. You can Read Online Handbook Of Single Molecule Fluorescence Spectroscopy here in PDF, EPUB, Mobi or Docx formats.

Handbook Of Single Molecule Fluorescence Spectroscopy

Author : Chris Gell
ISBN : 9780198529422
Genre : Science
File Size : 65. 51 MB
Format : PDF, ePub
Download : 456
Read : 650

Get This Book


This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Handbook Of Fluorescence Spectroscopy And Imaging

Author : Markus Sauer
ISBN : 9783527633524
Genre : Science
File Size : 85. 79 MB
Format : PDF, Kindle
Download : 485
Read : 1255

Get This Book


Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Handbook Of Single Molecule Biophysics

Author : Peter Hinterdorfer
ISBN : 0387764976
Genre : Science
File Size : 33. 30 MB
Format : PDF, ePub, Docs
Download : 470
Read : 1211

Get This Book


This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Single Molecule Fluorescence Spectroscopy Of The Folding Of A Repeat Protein

Author : Sharona Cohen
ISBN : 9783319095585
Genre : Science
File Size : 34. 13 MB
Format : PDF, Docs
Download : 955
Read : 1315

Get This Book


In this thesis single-molecule fluorescence resonance energy transfer (FRET) spectroscopy was used to study the folding of a protein that belongs to the large and important family of repeat proteins. Cohen shows that the dynamics of the expanded conformations is likely to be very fast, suggesting a spring-like motion of the whole chain. The findings shed new light on the elasticity of structure in repeat proteins, which is related to their function in binding multiple and disparate partners. This concise research summary provides useful insights for students beginning a PhD in this or a related area, and researchers entering this field.

Molecular Fluorescence

Author : Bernard Valeur
ISBN : 9783527650026
Genre : Science
File Size : 65. 28 MB
Format : PDF, Mobi
Download : 128
Read : 980

Get This Book


This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Principles Of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
ISBN : 9781461576587
Genre : Science
File Size : 24. 82 MB
Format : PDF, ePub, Docs
Download : 327
Read : 1292

Get This Book


Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Single Molecule Spectroscopy

Author : R. Rigler
ISBN : 9783642565441
Genre : Science
File Size : 55. 68 MB
Format : PDF
Download : 981
Read : 1158

Get This Book


The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Theory And Evaluation Of Single Molecule Signals

Author : Eli Barkai
ISBN : 9789812793492
Genre : Electronic books
File Size : 26. 19 MB
Format : PDF, ePub
Download : 574
Read : 275

Get This Book


This book reviews recently developed theoretical and numerical approaches to deal with optical and mechanical signals from individual molecules. The character of data generated by single molecules, and more generally by single nano-objects, qualitatively differs from those obtained in conventional experiments on large ensembles of molecules. Fluctuations, randomness and irreproducibility are central to single-molecule measurements, and the specific methods required to extract reliable and statistically relevant information from them are presented here. With contributions mainly from participants of the OC Theory, Modeling and Evaluation of Single-Molecule MeasurementsOCO workshop held in Leiden, the Netherlands, on April 16-20, 2007, this book is an authoritative compendium on the subject.

Single Molecule Biology

Author : Alexander E. Knight
ISBN : 008092123X
Genre : Science
File Size : 90. 51 MB
Format : PDF, Kindle
Download : 359
Read : 341

Get This Book


Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective. The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. * Organized by biological system or molecule. * Each chapter discusses insights into mechanism of action, structure, and function * Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc. * Includes an introduction to key principles and an historical overview. * Discusses applications in molecular diagnostics and drug discovery. * Provides an expert's perspective on future developments.

Single Molecule Science

Author : Dmitrii E. Makarov
ISBN : 9781466559523
Genre : Science
File Size : 84. 43 MB
Format : PDF, ePub, Docs
Download : 795
Read : 881

Get This Book


The observation and manipulation of individual molecules is one of the most exciting developments in modern molecular science. Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations. This book explains the physical principles underlying the major classes of single-molecule experiments such as fluorescence measurements, force-probe spectroscopy, and nanopore experiments. It provides the framework needed to understand single-molecule phenomena by introducing all the relevant mathematical and physical concepts, and then discussing various approaches to the problem of interpreting single-molecule data. The essential concepts used throughout this book are explained in the appendices and the text does not assume any background beyond undergraduate chemistry, physics, and calculus. Every effort has been made to keep the presentation self-contained and derive results starting from a limited set of fundamentals, such as several simple models of molecular dynamics and the laws of probability. The result is a book that develops essential concepts in a simple yet rigorous way and in a manner that is accessible to a broad audience.

Top Download:

Best Books