high performance liquid chromatography in plant sciences

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High Performance Liquid Chromatography In Plant Sciences

Author : Hans-Ferdinand Linskens
ISBN : 9783642829512
Genre : Science
File Size : 66. 96 MB
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Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.

High Performance Liquid Chromatography In Plant Science

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ISBN : OCLC:475453708
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File Size : 65. 25 MB
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High Performance Liquid Chromatography In Phytochemical Analysis

Author : Monika Waksmundzka-Hajnos
ISBN : 1420092618
Genre : Science
File Size : 81. 78 MB
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The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field. The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products. Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

High Performance Liquid Chromatography And Mass Spectrometry Of Porphyrins Chlorophylls And Bilins

Author : Chang Kee Lim
ISBN : 9789814497671
Genre : Science
File Size : 25. 41 MB
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The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field. Contents:Structure, Distribution, Biosynthesis and FunctionHigh-Performance Liquid Chromatography of PorphyrinsMass Spectrometry of PorphyrinsPorphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MSIsolation and Characterization of Protoporphyrin Glycoconjugates from Harderian Glands of Rodents by HPLC and HPLC/ESI-MSHPLC and HPLC/MS of Chlorophyll and Related CompoundsHPLC and HPLC/MS of Bilins of Animal and Plant OriginFuture Directions of HPLC and Mass spectrometry of Tetrapyrroles Readership: Analytical biochemists, clinical biochemists, researchers in tetrapyrolle chemistry and biochemistry, plant scientists, pharmaceutical chemists. keywords:Porphyrins;Chlorophylls;Bile Pigments;Bilins;High-Performance Liquid Chromatography of Porphyrins;Mass Spectrometry of Porphyrins;Tandem MS/MS of Tetrapyrroles

Plantomics The Omics Of Plant Science

Author : Debmalya Barh
ISBN : 9788132221722
Genre : Science
File Size : 43. 68 MB
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PlantOmics: The Omics of Plant Science provides a comprehensive account of the latest trends and developments of omics technologies or approaches and their applications in plant science. Thirty chapters written by 90 experts from 15 countries are included in this state-of-the-art book. Each chapter describes one topic/omics such as: omics in model plants, spectroscopy for plants, next generation sequencing, functional genomics, cyto-metagenomics, epigenomics, miRNAomics, proteomics, metabolomics, glycomics, lipidomics, secretomics, phenomics, cytomics, physiomics, signalomics, thiolomics, organelle omics, micro morphomics, microbiomics, cryobionomics, nanotechnology, pharmacogenomics, and computational systems biology for plants. It provides up to date information, technologies, and their applications that can be adopted and applied easily for deeper understanding plant biology and therefore will be helpful in developing the strategy for generating cost-effective superior plants for various purposes. In the last chapter, the editors have proposed several new areas in plant omics that may be explored in order to develop an integrated meta-omics strategy to ensure the world and earth’s health and related issues. This book will be a valuable resource to students and researchers in the field of cutting-edge plant omics.

Physical Methods In Plant Sciences

Author : Hans-Ferdinand Linskens
ISBN : 9783642836114
Genre : Science
File Size : 69. 45 MB
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A Practical Handbook Of Preparative Hplc

Author : Donald A Wellings
ISBN : 0080458858
Genre : Technology & Engineering
File Size : 69. 48 MB
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This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA. This book... •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems ·Provides practical, hands-on advice based on years of experience ·Will help ensure optimal design, equipment and separation results for your particular task ·Presents system layouts from laboratory to process scale ·Will help you to devise or improve record-keeping and documentation systems

Plant Cell Biology

Author : William V Dashek
ISBN : 1578083761
Genre : Science
File Size : 83. 35 MB
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While there are a few plant cell biology books that are currently available, these are expensive, methods-oriented monographs. The present volume is a textbook for "upper" undergraduate and beginning graduate students." This textbook stresses concepts and is inquiry-oriented. To this end, there is extensive use of original research literature. As we live in an era of literature explosion, one must be selective. These judgements will naturally vary with each investigator. Input was sought from colleagues in deciding the literature to include. In addition to provision of select research literature, this volume presents citations and summaries of certain laboratory methods. In this connection, the textbook stresses quantitative data to enhance the student?s analytical abilities. Thus the volume contains computer-spread sheets and references to statistical packages, e.g. Harvard Graphics and Statistica.

Issues In Life Sciences Botany And Plant Biology Research 2011 Edition

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ISBN : 9781464963438
Genre : Science
File Size : 38. 78 MB
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Issues in Life Sciences: Botany and Plant Biology Research: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Life Sciences—Botany and Plant Biology Research. The editors have built Issues in Life Sciences: Botany and Plant Biology Research: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Life Sciences—Botany and Plant Biology Research in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Life Sciences: Botany and Plant Biology Research: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

High Performance Liquid Chromatography Fingerprinting Technology Of The Commonly Used Traditional Chinese Medicine Herbs

Author : Baochang Cai
ISBN : 9789814291095
Genre : Medical
File Size : 34. 53 MB
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This book provides a comprehensive and up-to-date account of the HPLC technology and fingerprints of commonly-used Chinese herbs. It contains HPLC fingerprints of over 40 traditional Chinese medicinal herbs and details their sources, production and quality control. The book provides a practical review of techniques, relevant materials, and the particular demands for using HPLC in traditional Chinese medicinal herb applications. It also covers HPLC methods used to analyze the herbs. High-performance liquid chromatography (HPLC) is increasingly used in the field of traditional Chinese medicine for obtaining fingerprints of Chinese medicinal herbs. Advantages such as high speed, high resolution, high sensitivity, stability, accuracy, and automation make it one of the leading techniques used for quality control, standardization of planting, and new drug development in traditional Chinese medicine. To date, no other books on HPLC fingerprints of traditional Chinese medicinal herbs have been published in English. This is the first book in English devoted to providing readers with state-of-the-art information on HPLC applied in the analysis of traditional Chinese medicinal herbs.

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