key experiments in practical developmental biology

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Key Experiments In Practical Developmental Biology

Author : Manuel Marí-Beffa
ISBN : 0521833159
Genre : Science
File Size : 41. 7 MB
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This unique resource presents 27 laboratory exercises for use in student practical classes, all of which are classic experiments in developmental biology. These are experiments that provided key insights into developmental questions, and many of them are described by the scientists who carried out the original pioneering research. This book provides a bridge between state-of-the-art experimental work and the laboratory classes taken at the undergraduate and post-graduate levels. All chapters follow the same logical format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale employed in the research.

Developmental Biology Protocols

Author : Rocky S. Tuan
ISBN : 9781592590650
Genre : Science
File Size : 50. 17 MB
Format : PDF, Kindle
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The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.

Developmental Biology Of The Sea Urchin And Other Marine Invertebrates

Author : David J. Carroll
ISBN : 1493959026
Genre : Science
File Size : 56. 48 MB
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In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.

Egg And Ego

Author : J.M.W. Slack
ISBN : 038798559X
Genre : Biography & Autobiography
File Size : 44. 39 MB
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Egg and Ego is a book aimed at a general audience of student biologists. It is part a personal account of Jonathan Slack's own life in science (specifically developmental biology),and part an entertaining description and discussion of what it is like to a professional biologist. This superbly written book provides an intriguing (and often amusing) account of what exactly cellular and molecular biologists do in their jobs.

Practical Systems Biology

Author : Alistair Hetherington
ISBN : 9780415407809
Genre : Science
File Size : 38. 56 MB
Format : PDF, ePub
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Systems biology is the study of organisms as interacting networks of genes, proteins and reactions. Practical Systems Biology provides a detailed overview of the different approaches used in this relatively new discipline, integrating bioinformatics, genomics, proteomics and metabolomics. Various areas of research are also discussed, including the use of computational models of biological processes, and post-genomic research. Each chapter is written by an experienced researcher and gives an excellent account of various issues of systems biology that is suitable for postgraduate and postdoctoral researchers who are interested in this expanding area of science.

Die Evolution Der Kooperation

Author : Robert Axelrod
ISBN : 9783486851748
Genre : Philosophy
File Size : 58. 61 MB
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Wie ist Kooperation möglich in einer Welt von Egoisten, in der es keine zentrale Autorität gibt? Axelrod entwickelt aus der Spieltheorie - auf der Basis des "Gefangenendilemmas" - eine verblüffende Lösung. Nicht die raffinierteste, auch nicht die aggressivste, nicht die sanfteste Strategie sichert langfristig Erfolg, sondern die grundsätzlich wohlwollende, aber stets vergeltungsbereite: TIT FOR TAT, "wie Du mir, so ich Dir". Axelrod schließt daraus, dass in der globalen arbeitsteiligen Wirtschaftsweise geradezu ein innerer Zwang zur Kooperation besteht. Gewinnbringende Kooperationen sind für Unternehmen nützlicher als blinder Wettbewerb.

Johnson Volpe S Patterns Experiments In Developmental Biology

Author : Leland G. Johnson
ISBN : STANFORD:36105017321857
Genre : Science
File Size : 90. 22 MB
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A laboratory manual for developmental biology offering basic, easy to use, laboratory investigations (18 experiments) spanning various models including echinoderm (Sea Urchin), amphibian (Frog), chick embryo, and fern gametophyte.

Essential Developmental Biology

Author : Claudio D. Stern
ISBN : UOM:39015038998871
Genre : Law
File Size : 69. 6 MB
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Early Development Of Xenopus Laevis

Author : Hazel L. Sive
ISBN : 0879695781
Genre : Nature
File Size : 22. 32 MB
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Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Developmental Toxicology

Author : Craig Harris
ISBN : 1617798665
Genre : Medical
File Size : 29. 15 MB
Format : PDF, ePub
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The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology. The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development.

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