key experiments in practical developmental biology

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Key Experiments In Practical Developmental Biology

Author : Manuel Marí-Beffa
ISBN : 0521833159
Genre : Science
File Size : 73. 37 MB
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This unique resource presents 27 laboratory exercises for use in student practical classes, all of which are classic experiments in developmental biology. These are experiments that provided key insights into developmental questions, and many of them are described by the scientists who carried out the original pioneering research. This book provides a bridge between state-of-the-art experimental work and the laboratory classes taken at the undergraduate and post-graduate levels. All chapters follow the same logical format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale employed in the research.

Developmental Biology Of The Sea Urchin And Other Marine Invertebrates

Author : David J. Carroll
ISBN : 1493959026
Genre : Science
File Size : 52. 52 MB
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In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.

Laboratory Exercises In Developmental Biology

Author : Yolanda P. Cruz
ISBN : 9780323137775
Genre : Science
File Size : 28. 86 MB
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This intensive manual provides students with valuable information and insights into animal development at the organismal, cellular, and subcellular levels. The book uses both descriptive and investigative approaches that emphasize techniques, key experiments, and data analysis. Provides a broad introductory view of developmental systems Teaches both classical embryology and modern experimental approaches Contains seventeen laboratory exercises, written in step-by-step style Organized with additional notes to students and preparators Lists questions and references for each exercise Special chapters give introductions to the scientific process, use of the microscope, and the writing of scientific papers Illustrated with detailed line drawings

Experimental Developmental Biology

Author : Laura R. Keller
ISBN : 0124039707
Genre : Science
File Size : 64. 15 MB
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This work is designed for use as a lab manual in college-level courses in developmental biology or animal development. It could also be used in cell biology lab courses. In each laboratory exercise, students examine gametes and developing embryos of a single species, and also perform several experiments to probe the developmental process best illustrated by that species. A feature of this manual is the preparator's guide, which provides information for setting up each lab.

Developmental Biology Protocols

Author : Rocky S. Tuan
ISBN : 9781592590650
Genre : Science
File Size : 35. 43 MB
Format : PDF
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The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.

Principles Of Development

Author : Lewis Wolpert
ISBN : 9780198709886
Genre : Developmental biology
File Size : 37. 37 MB
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The process of biological development is an amazing feat of tightly regulated cellular behaviours--differentiation, movement, and growth--powerful enough to result in the emergence of a highly complex living organism from a single cell: the fertilized egg. Principles of Development clearlyillustrates the universal principles that govern this process of development in a succinct and accessible style. Cutting-edge science is explained clearly and succinctly, richly illustrated with a variety of custom drawn figures, animations, and online resources. A focus on the key principles of development throughout the text provides a framework on which a richer understanding of specific topics can bebuilt.

Imaging In Developmental Biology

Author : James Sharpe
ISBN : 087969940X
Genre : Science
File Size : 81. 90 MB
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Imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. This manual presents a comprehensive set of essential visualization methods.

Principles And Techniques Of Biochemistry And Molecular Biology

Author : Keith Wilson
ISBN : 9781107393493
Genre : Science
File Size : 86. 81 MB
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This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Early Development Of Xenopus Laevis

Author : Hazel L. Sive
ISBN : 0879695781
Genre : Nature
File Size : 61. 74 MB
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Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Johnson Volpe S Patterns Experiments In Developmental Biology

Author : Leland G. Johnson
ISBN : STANFORD:36105017321857
Genre : Science
File Size : 49. 33 MB
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A laboratory manual for developmental biology offering basic, easy to use, laboratory investigations (18 experiments) spanning various models including echinoderm (Sea Urchin), amphibian (Frog), chick embryo, and fern gametophyte.

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