methods in plant molecular biology

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Methods In Plant Molecular Biology And Biotechnology

Author : Bernard R. Glick
ISBN : UOM:39015029888370
Genre : Science
File Size : 46. 77 MB
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The use recombinant DNA technology to produce genetically modified plants; Production ans analysis of plant mutants, emphasizing arabidopsis thaliana; DNA sequence organization and gene copy number determination. Isolation and characterization of plant DNAs; Isolation and characterization of plant mRNA; Procedures for introducing foreign DNA into plants; Vectors for plant transformation; Construction of clone banks; Transient analysis of gene expression in plant cells; Techiniques for isolating and characterizing plant transcription promoters, enhancers, and terminators; Use of xenoplus oocytes to monitor plant geneexpression; In situ localization of specific mRNAs in plant tissues; Immunological methods for assessing protein expression in plants; In situ immunocytochemical localization of plant proteins; Accessing computer software for molecular biology; Sequence similarity searches, multiple sequence alignments, and molecular tree building; DNA mapping plants; Random amplified polymorphic DNA(RAPD) analysis; Detection and characterization of plant pathogens; Screening for inoculant-quality strains of rhizobia; Isolation and characterization of plant growth-promoting rhizobacteria.

Methods In Plant Molecular Biology

Author : Mary A. Schuler
ISBN : 9780323137652
Genre : Science
File Size : 60. 29 MB
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Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Eight basic experiments which can be used collectively or individually cover Recombinant Cloning and Screening in E. coli; DNA Sequencing Plant RNA Isolation and in Vitro Translations Plant DNA Isolations and Genomic DNA Southern Analysis Chloroplast Isolation and Protein Synthesis Plant Tissue Culture and Agrobacterium Transformations Experiments that have been student tested for three years Blueprints for setting up gel rigs Comprehensive course schedule outlining individual procedures to be finished in each lab segment Course can be tailored to suit the needs of the individual instructor

Methods In Plant Biochemistry And Molecular Biology

Author : William V. Dashek
ISBN : 0849394805
Genre : Science
File Size : 38. 52 MB
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Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

Methods For Plant Molecular Biology

Author : Arthur Weissbach
ISBN : 9781483269726
Genre : Science
File Size : 31. 10 MB
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Methods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and characterization of nuclear, chloroplast, and mitochondrial nucleic acids and the factors and systems involved in transcription and gene expression. Procedures for the isolation of cell walls, chloroplast membranes, membrane proteins; techniques to carry out plant cell culture and protoplast formation; and methods for gene and organelle transfer are covered as well. Biologists, molecular biologists, botanists, and students will find the book very useful.

Methods In Plant Molecular Biology

Author : P. Maliga
ISBN : UOM:39015053939826
Genre : Science
File Size : 26. 6 MB
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Plant Molecular Biology A Laboratory Manual

Author : Melody S. Clark
ISBN : 9783642878732
Genre : Science
File Size : 49. 38 MB
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Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.

Plant Molecular Biology Manual

Author : Stanton B. Gelvin
ISBN : 9789401154000
Genre : Science
File Size : 64. 95 MB
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Plant Cell Culture Protocols

Author : Victor M. Loyola-Vargas
ISBN : 9781592599592
Genre : Science
File Size : 30. 89 MB
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This second edition of Plant Cell Protocols follows a similar vein as its successful predecessor in providing an updated step=by-step guide to the most common, and applicable techniques and methods for plant tissue and cell culture. A total of 30 chapters, divided into 6 major sections have been included. Topics selected cover from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation, passing through the laborious process of protoplast isolation and culture. The protocols are currently used in the research programs of the authors, or represent important parts of business projects aimed at the generation of improved plant materials.

Molecular Biology Techniques

Author : Walt Ream
ISBN : 9780080536828
Genre : Science
File Size : 30. 28 MB
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Practical Applications Of Plant Molecular Biology

Author : Robert J. Henry
ISBN : 0412732203
Genre : Science
File Size : 69. 53 MB
Format : PDF, ePub
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Practical Applications of Plant Molecular Biology is an important new title which covers the major techniques and how they are applied to a range of vitally important areas. Divided broadly into four sections, this book covers key subjects including the identification of plants and plant pathogens using molecular techniques, the estimation of genetic variation in plants, the use of molecular markers in plant improvement and the use of plant transformation techniques for the improvement of quality and the introduction of resistance. Also included is a comprehensive listing and description of the most frequently used techniques and a set of appendices covering useful topics of reference for the reader. All undergraduates studying plant sciences, molecular biology, biotechnology and agricultural sciences would benefit from having access to this title as would those studying for upper-level Masters courses concentrating on the disciplines covered. This book also provides an invaluable source of reference for professionals in agriculture, plant breeding, crop protection and improvement, biotechnology and molecular biology.

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