modern hplc for practicing scientists

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Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 9781119293606
Genre : Science
File Size : 58. 27 MB
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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 9780471973096
Genre : Science
File Size : 36. 35 MB
Format : PDF, Docs
Download : 255
Read : 766

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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 047172789X
Genre : Science
File Size : 36. 55 MB
Format : PDF, Kindle
Download : 387
Read : 240

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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Introduction To Modern Liquid Chromatography

Author : Lloyd R. Snyder
ISBN : 9781118210390
Genre : Science
File Size : 31. 67 MB
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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : 9781118591512
Genre : Science
File Size : 22. 86 MB
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Hplc For Pharmaceutical Scientists

Author : Yuri V. Kazakevich
ISBN : 9780470087947
Genre : Science
File Size : 39. 19 MB
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HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

Mass Spectrometry For The Novice

Author : John Greaves
ISBN : 9781420094206
Genre : Science
File Size : 47. 57 MB
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With usage of mass spectrometry continually expanding, an increasing number of scientists, technicians, students, and physicians are coming into contact with this valuable technique. Mass spectrometry has many uses, both qualitative and quantitative, from analyzing simple gases to environmental contaminants, pharmaceuticals, and complex biopolymers. The extraordinary versatility can make mass spectrometers daunting to novices. Consequently, new users would benefit greatly from an understanding of the basic concepts as well as the processes that occur in these instruments. Mass Spectrometry for the Novice provides exactly that, with detailed, straightforward descriptions and clear illustrations of principles of operations and techniques. The book begins with an overview that includes essential definitions and then provides information on the components of and the strategies used in the most common instruments. The authors discuss the methodologies available, classes of compounds analyzed, and the types of data that can be generated. A group of representative applications from published articles is summarized, demonstrating the diversity of mass spectrometry. The authors also condense the essentials of the topic into one invaluable chapter that provides a set of concise take-home messages on all aspects of mass spectrometry. The final section provides a collection of resources including books, reviews, and useful websites. Using simple language, new color figures, clever cartoons, and assuming no prior knowledge, this book provides a readily understandable entrée to mass spectrometry. A CD-ROM with selected figures and cartoons is included.

Lc Ms

Author : Marvin C. McMaster
ISBN : 9780471736578
Genre : Science
File Size : 21. 9 MB
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A practical guide to using and maintaining an LC/MS system The combination of liquid chromatography (LC) and mass spectrometry (MS) has become the laboratory tool of choice for a broad range of industries that require the separation, analysis, and purification of mixtures of organic compounds. LC/MS: A Practical User's Guide provides LC/MS users with an easy-to-use, hands-on reference that focuses on the practical applications of LC/MS and introduces the equipment and techniques needed to use LC/MS successfully. Following a thorough explanation of the basic components and operation of the LC/MS system, the author presents empirical methods for optimizing the techniques, maintaining the instrumentation, and choosing the appropriate MS or LC/MS analyzer for any given problem. LC/MS covers everything users need to know about: The latest equipment, including quadrupole, time-of-flight, and ion trap analyzers Cutting-edge processes, such as preparing HPLC mobile phases and samples; handling and maintaining a wide variety of silica, zirconium, and polymeric separation columns; interpreting and quantifying mass spectral data; and using MS interfaces Current and future applications in the pharmaceutical and agrochemical industries, biotechnology, clinical research, environmental studies, and forensics An accompanying PowerPoint® slide-set on CD-ROM provides vital teaching tools for instructors and new equipment operators. Abundantly illustrated and easily accessible, the text is designed to help students and practitioners acquire optimum proficiency in this powerful and rapidly advancing analytical application.

Quantification In Lc And Gc

Author : Stavros Kromidas
ISBN : 9783527323012
Genre : Science
File Size : 52. 45 MB
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Closing a gap in the current literature by addressing the evaluation and quality assessment of raw data, this practice-oriented guide is clearly divided into three parts. The first describes basic considerations of chromatographic data quality, common errors and potential pitfalls in reading out and quantifying the data. Part two systematically covers the most important chromatographic methods as well as the specific requirements for obtaining good chromatographic data. The final part looks at data quality from the perspective of those regulatory authorities demanding certain standards in data quality, describing in detail best practices. Written with the practitioner in mind, the text not only teaches the mathematical basics but also provides invaluable advice.

Essentials In Modern Hplc Separations

Author : Serban C. Moldoveanu
ISBN : 9780123850133
Genre : Science
File Size : 45. 95 MB
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This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc. Gives a clear presentation of notions and conceptsDiscusses key parameters in HPLC separationIncludes modern developments in HPLCDescribes interrelation between various HPLC features (solvent pressure, separation, detection)Includes a large number of references.

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