modern hplc for practicing scientists

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Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 9780471973096
Genre : Science
File Size : 49. 80 MB
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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC forPracticing Scientists is a concise text which presents the mostimportant High-Performance Liquid Chromatography (HPLC)fundamentals, applications, and developments. It describes basictheory and terminology for the novice, and reviews relevantconcepts, best practices, and modern trends for the experiencedpractitioner. Moreover, the book serves well as an updatedreference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener"methods Regulatory aspects While broad in scope, this book focuses particularly onreversed-phase HPLC, the most common separation mode, and onapplications for the pharmaceutical industry, the largest usersegment. Accessible to both novice and intermedate HPLC users,information is delivered in a straightforward manner illustratedwith an abundance of diagrams, chromatograms, tables, and casestudies, and supported with selected key references and Webresources. With intuitive explanations and clear figures, Modern HPLC forPracticing Scientists is an essential resource for practitioners ofall levels who need to understand and utilize this versatileanalytical technology.

Author : مارك مغربى كيرلانسكى
ISBN : 9990601739
Genre : Salt
File Size : 41. 15 MB
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Essentials In Modern Hplc Separations

Author : Serban C. Moldoveanu
ISBN : 9780123850140
Genre : Science
File Size : 39. 30 MB
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Essentials in Modern HPLC Separations discusses the role of separation in high performance liquid chromatography (HPLC). This up-to-date reference systematically covers new developments in types and characteristics of stationary phases, mobile phases, and other factors of this technique that influence separation of compounds being analyzed. The volume also considers the selection process for stationary and mobile phases in relation to the molecules being separated and examined, as well as their matrices. The book includes a section on the contemporary applications of HPLC, particularly the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, and more. Discusses key parameters in HPLC separation Describes interrelation between various HPLC features (solvent pressure, separation, detection) Includes a large number of references

Analysis And Analyzers

Author : Béla G. Lipták
ISBN : 9781315353241
Genre : Science
File Size : 55. 97 MB
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The Instrument and Automation Engineers’ Handbook (IAEH) is the #1 process automation handbook in the world. Volume two of the Fifth Edition, Analysis and Analyzers, describes the measurement of such analytical properties as composition. Analysis and Analyzers is an invaluable resource that describes the availability, features, capabilities, and selection of analyzers used for determining the quality and compositions of liquid, gas, and solid products in many processing industries. It is the first time that a separate volume is devoted to analyzers in the IAEH. This is because, by converting the handbook into an international one, the coverage of analyzers has almost doubled since the last edition. Analysis and Analyzers: Discusses the advantages and disadvantages of various process analyzer designs Offers application- and method-specific guidance for choosing the best analyzer Provides tables of analyzer capabilities and other practical information at a glance Contains detailed descriptions of domestic and overseas products, their features, capabilities, and suppliers, including suppliers’ web addresses Complete with 82 alphabetized chapters and a thorough index for quick access to specific information, Analysis and Analyzers is a must-have reference for instrument and automation engineers working in the chemical, oil/gas, pharmaceutical, pollution, energy, plastics, paper, wastewater, food, etc. industries. About the eBook The most important new feature of the IAEH, Fifth Edition is its availability as an eBook. The eBook provides the same content as the print edition, with the addition of thousands of web addresses so that readers can reach suppliers or reference books and articles on the hundreds of topics covered in the handbook. This feature includes a complete bidders' list that allows readers to issue their specifications for competitive bids from any or all potential product suppliers.

Liquid Chromatography

Author : J.G. Shackman
ISBN : 9780128071403
Genre : Science
File Size : 87. 60 MB
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This chapter provides an overview of the most common equipment used for performing high-pressure liquid chromatography (HPLC), under both conventional (up to 6000 psi; 400 bar) and very high pressure conditions (6,000 –20,000 psi; 400-1400 bar), including: pumps, sample injectors, column compartments, and connections. The operation and figures of merit of the most frequently encountered detectors (absorbance, refractive index, nanoparticle based, conductivity, fluorescence, and electrochemical) are also described. Practical tips and considerations for achieving optimal chromatography are provided throughout.

Hplc For Pharmaceutical Scientists

Author : Yuri V. Kazakevich
ISBN : 9780470087947
Genre : Science
File Size : 82. 8 MB
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HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

Hydrophilic Interaction Chromatography

Author : Bernard A. Olsen
ISBN : 1118054172
Genre : Science
File Size : 27. 69 MB
Format : PDF
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Discover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

European Journal Of Organic Chemistry

Author :
ISBN : CHI:81573008
Genre : Bioorganic chemistry
File Size : 45. 73 MB
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Liquid Chromatography

Author : Salvatore Fanali
ISBN : 9780128093443
Genre : Science
File Size : 64. 59 MB
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Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Practice Of High Performance Liquid Chromatography

Author : Heinz Engelhardt
ISBN : 9783642692253
Genre : Science
File Size : 77. 25 MB
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During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

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