optical fluorescence microscopy from the spectral to the nano dimension

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Optical Fluorescence Microscopy

Author : Alberto Diaspro
ISBN : 3642151752
Genre : Science
File Size : 77. 34 MB
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In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Bilder Der Pr Zision

Author : Matthias Bruhn
ISBN : 9783110417081
Genre : Art
File Size : 62. 57 MB
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Präzision ist ein Schlüsselbegriff in Technik, Kunst und Wissenschaft, der in enger Verbindung mit Verfahren der Messung und Feinmechanik steht, aber auch die Schärfe sprachlicher Begriffsbildung oder die Synchronisation tänzerischer Bewegungen beschreiben kann. Die Wissenschaftsgeschichte zeigt, dass der Bedarf nach größtmöglicher Präzision auch die Widersprüche von Messung und Modell, Versuch und Vorhersage in sich aufgenommen hat – abweichende Messergebnisse wurden so zum eigentlichen Beleg für die Genauigkeit einer Methode und den Bedarf ihrer weiteren Raffinierung. Der interdisziplinäre Blick auf verschiedene Felder zeigt, wie der damit verbundene Anspruch in ihnen produktiv gemacht und reflektiert worden ist. Fallstudien zu Beispielen aus mehreren Jahrhunderten gehen dieser Frage nach, ergänzt um Auszüge aus grundlegenden historisch-theoretischen Beiträgen.

Romeis Mikroskopische Technik

Author : Maria Mulisch
ISBN : 9783642551901
Genre : Science
File Size : 59. 55 MB
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Der ROMEIS ist seit fast 100 Jahren das Standardwerk der mikroskopischen Technik. Über 18 Auflagen hat dieses Methodenbuch die Entwicklung der lichtmikroskopischen Verfahren begleitet und ist bis heute ein unverzichtbares Laborhandbuch für Wissenschaftler und Studierende, die auf den Gebieten der Cytologie, Histologie, mikroskopischen Anatomie, Pathologie und Histochemie forschen. Der Inhalt der 19. Auflage des ROMEIS wurde aktualisiert und um viele moderne Methoden und Anwendungen der Mikroskopie erweitert. Unter der Herausgeberschaft von Privatdozentin Dr. Maria Mulisch und Professor Dr. med. Ulrich Welsch haben 24 Experten der Mikroskopie aus Forschung und Industrie ihre Erfahrung eingebracht, um dieses Werk zu einem Arbeitsbuch zu machen, auf das man sich beziehen und verlassen kann.

Fundamentals Of Fluorescence Microscopy

Author : Partha Pratim Mondal
ISBN : 9789400775459
Genre : Science
File Size : 39. 15 MB
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This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Nanotechnology In Catalysis 3 Volumes

Author : Bert Sels
ISBN : 9783527339143
Genre : Science
File Size : 88. 49 MB
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Reflecting the R&D efforts in the field that have resulted in a plethora of novel applications over the past decade, this handbook gives a comprehensive overview of the tangible benefits of nanotechnology in catalysis. By bridging fundamental research and industrial development, it provides a unique perspective on this scientifically and economically important field. While the first three parts are devoted to preparation and characterization of nanocatalysts, the final three provide in-depth insights into their applications in the fine chemicals industry, the energy industry, and for environmental protection, with expert authors reporting on real-life applications that are on the brink of commercialization. Timely reading for catalytic chemists, materials scientists, chemists in industry, and process engineers.

Chemical Imaging Analysis

Author : Freddy Adams
ISBN : 9780444634504
Genre : Science
File Size : 45. 53 MB
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Chemical Imaging Analysis covers the advancements made over the last 50 years in chemical imaging analysis, including different analytical techniques and the ways they were developed and refined to link the composition and structure of manmade and natural materials at the nano/micro scale to the functional behavior at the macroscopic scale. In a development process that started in the early 1960s, a variety of specialized analytical techniques was developed – or adapted from existing techniques – and these techniques have matured into versatile and powerful tools for visualizing structural and compositional heterogeneity. This text explores that journey, providing a general overview of imaging techniques in diverse fields, including mass spectrometry, optical spectrometry including X-rays, electron microscopy, and beam techniques. Provides comprehensive coverage of analytical techniques used in chemical imaging analysis Explores a variety of specialized techniques Provides a general overview of imaging techniques in diverse fields

Nano Optics And Near Field Optical Microscopy

Author : Anatoly V. Zayats
ISBN : 9781596932845
Genre : Electronic books
File Size : 88. 12 MB
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This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities.

Energy Materials Coordinating Committe Emacc Fiscal Year 2004 Annual Technical Report

Author :
ISBN : 9781422345597
Genre :
File Size : 33. 79 MB
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Comprehensive Nanoscience And Technology

Author :
ISBN : 0123743966
Genre : Science
File Size : 59. 81 MB
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From the Introduction: Nanotechnology and its underpinning sciences are progressing with unprecedented rapidity. With technical advances in a variety of nanoscale fabrication and manipulation technologies, the whole topical area is maturing into a vibrant field that is generating new scientific research and a burgeoning range of commercial applications, with an annual market already at the trillion dollar threshold. The means of fabricating and controlling matter on the nanoscale afford striking and unprecedented opportunities to exploit a variety of exotic phenomena such as quantum, nanophotonic and nanoelectromechanical effects. Moreover, researchers are elucidating new perspectives on the electronic and optical properties of matter because of the way that nanoscale materials bridge the disparate theories describing molecules and bulk matter. Surface phenomena also gain a greatly increased significance; even the well-known link between chemical reactivity and surface-to-volume ratio becomes a major determinant of physical properties, when it operates over nanoscale dimensions. Against this background, this comprehensive work is designed to address the need for a dynamic, authoritative and readily accessible source of information, capturing the full breadth of the subject. Its six volumes, covering a broad spectrum of disciplines including material sciences, chemistry, physics and life sciences, have been written and edited by an outstanding team of international experts. Addressing an extensive, cross-disciplinary audience, each chapter aims to cover key developments in a scholarly, readable and critical style, providing an indispensible first point of entry to the literature for scientists and technologists from interdisciplinary fields. The work focuses on the major classes of nanomaterials in terms of their synthesis, structure and applications, reviewing nanomaterials and their respective technologies in well-structured and comprehensive articles with extensive cross-references. It has been a constant surprise and delight to have found, amongst the rapidly escalating number who work in nanoscience and technology, so many highly esteemed authors willing to contribute. Sharing our anticipation of a major addition to the literature, they have also captured the excitement of the field itself in each carefully crafted chapter. Along with our painstaking and meticulous volume editors, full credit for the success of this enterprise must go to these individuals, together with our thanks for (largely) adhering to the given deadlines. Lastly, we record our sincere thanks and appreciation for the skills and professionalism of the numerous Elsevier staff who have been involved in this project, notably Fiona Geraghty, Megan Palmer and Greg Harris, and especially Donna De Weerd-Wilson who has steered it through from its inception. We have greatly enjoyed working with them all, as we have with each other.

Tip Enhanced Raman Spectroscopy For Nanoelectronics

Author : Mischa Nicklaus
ISBN : 9783732292783
Genre : Science
File Size : 53. 22 MB
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This dissertation focuses on the application of Tip-Enhanced Raman spectroscopy (TERS) to non-transparent and non-conductive samples, allowing for the optical characterization of nanoelectronic devices. As such, nano-crystals are analyzed as a model system for the investigation of chemical and structural properties. Furthermore, a novel method for mapping the refractive index of materials with nanometer resolution is presented. The technological progress of electronics through miniaturization has reached the nanoscale while new materials with high performance and functional properties gain importance. Quality control and the scientific understanding of size effects in electronic nanostructures are required more than ever to consolidate existing technologies and to determine scaling limits of new materials. Conventional techniques, including scanning electron and scanning probe microscopy, provide topographic information but only very limited chemical information to analyze the physical properties of nanomaterials. Chemical and structural sensitivity is available by Raman or infrared spectroscopy, but with a spatial resolution limited to the microscale by the diffraction limit of light. TERS combines the virtues of scanning probe microscopy with those of optical spectroscopy to overcome the diffraction limit through the excitation of surface plasmons on a scanning probe tip to confine light to nanometers. In this work, a TERS system was installed to operate on opaque samples by employing optical side access. TERS probes were fabricated by electrochemical etching and operated in scanning tunneling microscopy and atomic force microscopy with quartz tuning forks to enable scanning on various surfaces. TERS was then applied to ferroelectric lead titanate nano-crystals on a platinized silicon substrate as a model system for nanostructured, charge-based memory devices at the onset of finite size effects.

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