practical hplc method development

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Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : 9781118591512
Genre : Science
File Size : 55. 47 MB
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : 047100703X
Genre : Science
File Size : 26. 91 MB
Format : PDF, ePub, Mobi
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : UCSD:31822002498277
Genre : High performance liquid chromatography
File Size : 67. 35 MB
Format : PDF
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Getting started; Basics of separation: mobile-hase effects; The role of the column; Systematic method development; Difficult separations: the use of other separation variables and procedures; Gradient elution; Special samples and techniques; Computer-assisted method development; Method-development procedures.

Analytical Method Development And Validation

Author : Michael E. Swartz
ISBN : 9781482229776
Genre : Science
File Size : 25. 16 MB
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Describes analytical methods development, optimization and validation, and provides examples of successful methods development and validation in high-performance liquid chromatography (HPLC) areas. The text presents an overview of Food and Drug Administration (FDA)/International Conference on Harmonization (ICH) regulatory guidelines, compliance with validation requirements for regulatory agencies, and methods validation criteria stipulated by the US Pharmacopia, FDA and ICH.

Introduction To Modern Liquid Chromatography

Author : Lloyd R. Snyder
ISBN : 9781118210390
Genre : Science
File Size : 36. 15 MB
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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Hplc Method Development For Pharmaceuticals

Author : Satinder Ahuja
ISBN : 9780080554198
Genre : Medical
File Size : 84. 75 MB
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High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective. HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge. Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities) Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

Modern Hplc For Practicing Scientists

Author : Michael W. Dong
ISBN : 9781119293606
Genre : Science
File Size : 71. 11 MB
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A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Selection Of The Hplc Method In Chemical Analysis

Author : Serban C. Moldoveanu
ISBN : 9780128037119
Genre : Science
File Size : 50. 28 MB
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Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation. Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field Provides criteria for HPLC method selection, development, and validation Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field

Practical Hplc Methodology And Applications

Author : Brian A. Bidlingmeyer
ISBN : 0471572462
Genre : Science
File Size : 31. 82 MB
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Of related interest. Trace and Ultratrace Analysis by HPLC Satinder Ahuja Written by a leading scientist in the field, this monograph provides the first definitive and technically up–to–date treatment of the theory, equipment, and applications of chemistry′s most powerful reliable analytical technique. Coverage includes an encyclopedic compendium of common substances that require trace and ultratrace analysis, and features clear discussion of such important topics as considerations for HPLC equipment, sensitive detectors, sample preparation, method development, selectivity and computer–based optimizations, optimizing detectability, and much more. 1991 (0 471–51419–5) 432 pp. High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock Analytical chemists, biochemists, and chemical engineers will find this up–to–date guide to HPLC′s recent developments essential for enhancing on–the–job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as those in monoclonal antibody and nucleic acid purification. Techniques for quality control programs and advanced technology are also discussed. 1990 (0 471–82584–0) 564 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together for the first time the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physical–mathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide background material applicable to a wide range of separation methods, while the final five chapters illustrate specific techniques and methods. 1991 (0 471–52089–6) 320 pp.

Handbook Of Analytical Validation

Author : Michael E. Swartz
ISBN : 9781420014488
Genre : Science
File Size : 47. 9 MB
Format : PDF, Kindle
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Written for practitioners in both the drug and biotechnology industries, the Handbook of Analytical Validation carefully compiles current regulatory requirements on the validation of new or modified analytical methods. Shedding light on method validation from a practical standpoint, the handbook: Contains practical, up-to-date guidelines for analytical method validation Summarizes the latest regulatory requirements for all aspects of method validation, even those coming from the USP, but undergoing modifications Covers development, optimization, validation, and transfer of many different types of methods used in the regulatory environment Simplifying the overall process of method development, optimization and validation, the guidelines in the Handbook apply to both small molecules in the conventional pharmaceutical industry, as well as well as the biotech industry.

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