quantitative phase imaging of cells and tissues mcgraw hill biophotonics

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Quantitative Phase Imaging Of Cells And Tissues

Author : Gabriel Popescu
ISBN : 9780071663434
Genre : Technology & Engineering
File Size : 28. 20 MB
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Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI

Introduction To Optical Microscopy

Author : Jerome Mertz
ISBN : 0981519482
Genre : Technology & Engineering
File Size : 76. 91 MB
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Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, as it includes a corresponding website that provides problem sets and an instructor's solution manual.

Biomedical Applications Of Light Scattering

Author : Adam Wax
ISBN : 9780071598811
Genre : Medical
File Size : 53. 97 MB
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Clinical applications include: detecting pre-cancerous and cancerous tissue states; characterizing cell and tissue properties for identifying disease; and assessing the presence and concentration of biochemicals for diagnostic purposes Part of the McGraw-Hill Biophotonics Series

A Laboratory Manual In Biophotonics

Author : Vadim Backman
ISBN : 9781498744331
Genre : Medical
File Size : 73. 67 MB
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The increasing use of optical techniques for biomedical applications has led to significant interest in biophotonics as a growing field. From its basic concepts to more advanced topics and applications, this book provides a complete guide to biophotonics. The author offers both descriptive and quantitative perspectives as well as a comprehensive overview of major areas of research in the field. The text includes coverage of topics such as light tissue interaction, optical instrumentation, microscopic and macroscopic tissue imaging, tissue spectroscopy, therapeutic applications of optics, and molecular imaging.

Understanding Biophotonics

Author : Kevin Tsia
ISBN : 9789814411783
Genre : Medical
File Size : 21. 88 MB
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Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies. Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis. This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.

Annabelle Aiden

Author : Joseph Raphael Becker
ISBN : 0997806613
Genre : Juvenile Nonfiction
File Size : 53. 17 MB
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In this "delightful illustrated book written in rhyme" (as endorsed by Lawrence Krauss), Annabelle asks "Why do we look, the way that we do? With hands and feet, in neat sets of two? What made my eyes? And what made my nose? And the shape of my body, from my head to my toes?" A wise owl answers by taking the characters on an incredible journey through Darwinian evolution. Join our characters as they visit outer space, watch the Earth go through its earliest stages, and gaze in wonder at the earliest forms of life. Young readers will gain a basic understanding of evolution, and perhaps more importantly, what we can learn from it: to be kind to one another, as we are all related in the same family tree. To hear about future Annabelle & Aiden releases & news, 'like' our Facebook page at Facebook.com/AnnabelleAndAiden

Biophotonics

Author : Matthew Baker
ISBN : 9781681741352
Genre : Science
File Size : 24. 47 MB
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Biophotonic diagnostics/biomedical spectroscopy can revolutionise the medical environment by providing a responsive and objective diagnostic environment. This book aims to explain the fundamentals of the physical techniques used combined with the particular requirements of analysing medical/clinical samples as a resource for any interested party. In addition, it will show the potential of this field for the future of medical science and act as a driver for translation across many different biological problems/questions.

Biomedical Optical Phase Microscopy And Nanoscopy

Author : Natan T. Shaked
ISBN : 9780124158719
Genre : Medical
File Size : 69. 80 MB
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Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.

Second Harmonic Generation Imaging

Author : Francesco S. Pavone
ISBN : 9781439849156
Genre : Science
File Size : 55. 44 MB
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Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical applications. The book features contributions by experts in second-harmonic imaging, including many pioneering researchers in the field. Written for researchers at all levels, it takes an in-depth look at the current state of the art and possibilities of SHG microscopy. Organized into three sections, the book: Provides an introduction to the physics of the process, step-by-step instructions on how to build an SHG microscope, and comparisons with related imaging techniques Gives an overview of the capabilities of SHG microscopy for imaging tissues and cells—including cell membranes, muscle, collagen in tissues, and microtubules in live cells—by summarizing experimental and analytical methods Highlights representative biomedical and medical applications in imaging cancer, fibroses, autoimmune diseases, connective tissue disorders, eye pathologies, and cardiovascular disease Historically, clinical imaging at the cellular and tissue level has been performed by pathologists on ex vivo biopsies removed by the surgeon. While histology remains the "gold standard" for pathologists, its interpretation remains highly subjective. Much of SHG research has focused on developing more quantitative, objective metrics. A tutorial for newcomers and an up-to-date review for experts, this book explores how SHG may be used to more precisely image a wide range of pathological conditions and diseases.

Introduction To Biophotonics

Author : Paras N. Prasad
ISBN : 9780471465393
Genre : Science
File Size : 69. 45 MB
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Paras Prasad’s text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy Each section also includes illustrated examples and review questions to test and advance the reader’s knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.

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