the resolution revolution recent advances in cryoem methods in enzymology

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The Resolution Revolution Recent Advances In Cryoem

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ISBN : 9780128054352
Genre : Medical
File Size : 29. 10 MB
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cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their function Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Electron Crystallography Of Biological Macromolecules

Author : Robert M. Glaeser
ISBN : 0195088719
Genre : Science
File Size : 51. 5 MB
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This book provides a complete introduction to both the practical details and the theoretical foundations required in order to use electron microscopy as a research tool in structural biology. Planned and written by a group of 5 well-known experts who have pioneered different aspects of the field of electron cryo-microscopy (cryo-EM) of biological macromolecules, this book offers a depth of knowledge and expertise that could only be replicated from the primary literature with great difficulty. Figures and illustrations are used liberally to simplify the understanding of concepts and to provide ready access to examples of biological applications. In addition, numerous boxes provide auxiliary information for those who wish to go more deeply into areas of background with which they may not already be familiar. The organization of topics lends itself well to using this book either to gain a comprehensive mastery of all aspects of the field of cryo-EM or as an authoritative reference source for individual topics within the field.

Molecular Characterization Of Autophagic Responses

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ISBN : 9780128097953
Genre : Medical
File Size : 58. 81 MB
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Molecular Characterization of Autophagic Responses, Part A, presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses Written in an accessible style by renowned experts in the field

Single Particle Cryo Electron Microscopy

Author : Joachim Frank
ISBN : 9813234857
Genre : Science
File Size : 35. 15 MB
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Electron Crystallography

Author : D. Dorset
ISBN : 9789401589710
Genre : Science
File Size : 24. 84 MB
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The re-emergent field of quantitative electron crystallography is described by some of its most eminent practitioners. They describe the theoretical framework for electron scattering, specimen preparation, experimental techniques for optimum data collection, the methodology of structure analysis and refinement, and a range of applications to inorganic materials (including minerals), linear polymers, small organic molecules (including those used in nonlinear optical devices), incommensurately modulated structures (including superconductors), alloys, and integral membrane proteins. The connection between electron crystallography and X-ray crystallography is clearly defined, especially in the utilisation of the latest methods for direct determination of crystallographic phases, as well as the unique role of image analysis of high-resolution electron micrographs for phase determination. Even the aspect of multiple beam dynamic diffraction (once dreaded because it was thought to preclude ab initio analysis) is considered as a beneficial aid for symmetry determination as well as the elucidation of crystallographic phases, and as a criterion for monitoring the progress of structure refinement. Whereas other texts have hitherto preferentially dealt with the analysis of electron diffraction and image data from thin organic materials, this work discusses - with considerable optimism - the prospects of looking at `harder' materials, composed of heavier atoms. Audience: Could be used with profit as a graduate-level course on electron crystallography. Researchers in the area will find a statement of current progress in the field.

Three Dimensional Electron Microscopy Of Macromolecular Assemblies

Author : Joachim Frank
ISBN : 9780190292881
Genre : Science
File Size : 43. 27 MB
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Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

Dna Repair Enzymes Structure Biophysics And Mechanism

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ISBN : 9780128125168
Genre : Medical
File Size : 86. 95 MB
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DNA Repair Enzymes, Part B, Volume 592 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA Repair Enzymes. Topics in this updated volume include MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes, Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes, Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR, the Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair, and Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes. Includes contributions from leading authorities working in enzymology Focuses on DNA repair enzymes Informs and updates on all the latest developments in the field of enzymology

4d Electron Microscopy

Author : Ahmed H. Zewail
ISBN : 9781848164000
Genre : Science
File Size : 47. 51 MB
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Structural phase transitions, mechanical deformations, and the embryonic stages of melting and crystallization are examples of phenomena that can now be imaged in unprecedented structural detail with high spatial resolution, and ten orders of magnitude as fast as hitherto. No monograph in existence attempts to cover the revolutionary dimensions that EM in its various modes of operation nowadays makes possible. The authors of this book chart these developments, and also compare the merits of coherent electron waves with those of synchrotron radiation. They judge it prudent to recall some important basic procedural and theoretical aspects of imaging and diffraction so that the reader may better comprehend the significance of the new vistas and applications now afoot. This book is not a vade mecum - numerous other texts are available for the practitioner for that purpose.

Liquid Cell Electron Microscopy

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ISBN : 9781107116573
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Cellular Imaging

Author : Eric Hanssen
ISBN : 9783319689975
Genre : Science
File Size : 83. 66 MB
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This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

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