tissue culture methods and applications

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Tissue Culture

Author : Paul F. Jr. Kruse
ISBN : 9780323142076
Genre : Science
File Size : 80. 64 MB
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Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Plant Tissue Culture

Author : Gerard Meurant
ISBN : 9780323139434
Genre : Technology & Engineering
File Size : 62. 4 MB
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Plant Tissue Culture: Methods and Applications in Agriculture contains the proceedings of a symposium based on the UNESCO training course on Plant Tissue Culture: Methods and Applications in Agriculture, sponsored by UNESCO and held in Campinas, Sao Paulo, Brazil, on November 8-22, 1978. This book contains two major sections encompassing plant tissue culture: Part A, which focuses on methodology, and Part B, which emphasizes the applications. The first chapters present the requirements for a tissue culture facility, and then describe nutrition, media, and characteristics of cultured plant cells and their growth and behavior in vitro, particularly with reference to embryogenesis and organogenesis. Discussions on protoplasts, mutagenesis and in vitro selection, meristem culture, freeze preservation, and cytogenetic techniques complete Part A. In Part B, androgenesis, in vitro fertilization, and embryo culture are discussed. Some chapters follow on the application of in vitro methodology to selected crops. The final chapter deals with the potential of tissue culture in the biosynthesis of secondary products. This text will prove useful to those who must thoroughly plan their research in tackling problems in agriculture that are amenable to the tissue culture approach.

Plant Tissue Culture

Author : Trevor A. Thorpe
ISBN : 0126906807
Genre : Technology & Engineering
File Size : 25. 89 MB
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Requirements for a tissue culture facility. Nutrition, media, and characteristics of plant cell and tissue cultures. Growth and behavior of cell cultures: embryogenesis and organogenesis. Isolation, fusion, and culture of plant protoplasts. Mutagenesis and in vitro selection. Meristem culture and cryopreservation - methods and applications. Cytogenetic techniques. Production of isogenic lines: basic technical aspects of androgenesis. In vitro fertilization and embryo culture. In vitro methods applied to rice. In vitro methods applied to sugar cane improvement. In vitro methods applied to coffee. In vitro methods applied to forest trees. Biosynthesis of secondary products in vitro.

Experiments In Plant Tissue Culture

Author : John H. Dodds
ISBN : 0521315166
Genre : Science
File Size : 76. 48 MB
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The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture

Author : Timir Baran Jha
ISBN : 8173714886
Genre : Plant tissue culture
File Size : 75. 7 MB
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Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed.

Plant Tissue Culture And Moelcular Markers

Author : Ashwani Kumar
ISBN : 9788189866105
Genre : Agricultural productivity
File Size : 90. 90 MB
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Besides, recently molecular biology has assumed great importance with respect to plant biotechnology. The present book amalgamates all three aspects into one, practical applications of various techniques being the need of the hour. It discusses micropropagation studies on several crop plants, molecular basis of understanding various life processes including molecular basis of somatic embryogenesis and other physiological and biochemical processes having significant biotechnological applications. It also includes in vitro studies of some important plants like Aloe vera, Simmondsia chinensis, Anacyclus pyrethrum and Crataeva nurvala, Arachis hypogaea L., Phoenix dactylifera, Dendrocalamus asper, Asparagus adescendens Roxb., natural products of plant origin with their therapeutic potential and biotechnological production, genome analysis of crop plants with future applications in biotechnology etc.

Plant Tissue Culture

Author : S.S. Bhojwani
ISBN : 9780444598479
Genre : Science
File Size : 37. 72 MB
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During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly. This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.

Cell Culture

Author : William B. Jakoby
ISBN : 0121819582
Genre : Science
File Size : 75. 50 MB
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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Key Features * Covers basics in great detail * Laboratory Requirements and Media Cell Culture Techniques: Monolayers, Growth Measurement, and Mass Culture

Plant Tissue Culture And Its Agricultural Applications

Author : Lyndsey A. Withers
ISBN : 9781483192055
Genre : Technology & Engineering
File Size : 43. 44 MB
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Plant Tissue Culture and Its Agricultural Applications presents the proceedings of the 41st University of Nottingham Easter School in Agricultural Science held in England. The sessions covered in this volume reflect the revolution of tissue culture and its role in the propagation of elite plant material and the development of improved genotypes. This book is organized into four main sections. The first section chronicles the revolution of the plant tissue culture. This includes papers on clonal propagation, morphogenesis, germplasm storage, plant health, and genetic improvement. The core of this volume is covered by the introductory and the final chapters which interrelate the different subjects areas covered by the proceedings and provide a realistic assessment of future research required for the plant tissue culture revolution to come to fruition. This book will be useful to readers interested in understanding the history, evolution, and future of plant tissue culture and its applications in the agricultural sector.

Tierische Zellkulturen

Author : R. Ian Freshney
ISBN : 9783110889024
Genre : Science
File Size : 28. 66 MB
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