topics in fluorescence spectroscopy vol 5 nonlinear and two photon induced fluorescence

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Topics In Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
ISBN : 9780306470707
Genre : Science
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Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.

Topics In Fluorescence Spectroscopy Principles

Author : Joseph R. Lakowicz
ISBN : LCCN:91032671
Genre : Fluorescence spectroscopy
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Principles Of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
ISBN : 9781475730616
Genre : Science
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`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Topics In Fluorescence Spectroscopy Nonlinear And Two Photon Induced Fluoresence

Author : Joseph R. Lakowicz
ISBN : LCCN:91032671
Genre : Fluorescence spectroscopy
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Molecular Imaging

Author : Ammasi Periasamy
ISBN : 0080536875
Genre : Science
File Size : 85. 62 MB
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The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

International Conference On Lasers For Measurements And Information Transfer

Author : Vadim Evgenʹevich Privalov
ISBN : UOM:39015049972873
Genre : Science
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Two Proton Induced Fluorescence Of Artery Tissue

Author : Beth Michelle Mammini
ISBN : UCAL:X59447
Genre :
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Atomic And Molecular Spectroscopy

Author : Sune Svanberg
ISBN : 3540674322
Genre : Medical
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This successful reference presents a wide-ranging review of modern spectroscopic techniques such as X-ray, photoelectron, optical and laser spectroscopy, and related techniques. It focuses on physical principles and the impact of spectroscopy on our understanding of the building blocks of matter, while examining applications to chemical analysis, photochemistry, surface characterization, environmental and medical diagnostics, remote sensing, and astrophysics. This Third Edition includes the most up-to-date developments.

Jahrbuch

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ISBN : NWU:35556030679500
Genre : Science
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Nonlinear Optical Microscopy

Author : Romedi Selm
ISBN : 3843906661
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