ultra high performance liquid chromatography and its applications

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Ultra High Performance Liquid Chromatography And Its Applications

Author : Q. Alan Xu
ISBN : 9781118533987
Genre : Technology & Engineering
File Size : 66. 93 MB
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Explores both the benefits and limitations of new UHPLCtechnology High performance liquid chromatography (HPLC) has been widelyused in analytical chemistry and biochemistry to separate,identify, and quantify compounds for decades. The science of liquidchromatography, however, was revolutionized a few years ago withthe advent of ultra-high performance liquid chromatography (UHPLC),which made it possible for researchers to analyze sample compoundswith greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and ItsApplications enables readers to maximize the performance ofUHPLC as well as develop UHPLC methods tailored to their particularresearch needs. Readers familiar with HPLC methods will learn howto transfer these methods to a UHPLC platform and vice versa. Inaddition, the book explores a variety of UHPLC applicationsdesigned to support research in such fields as pharmaceuticals,food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development andmethod transfer between HPLC and UHPLC platforms. It then examinespractical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drugmetabolism and pharmacokinetics studies for traditional Chinesemedicine. With its frank discussions of UHPLC's benefits and limitations,Ultra-High Performance Liquid Chromatography and ItsApplications equips analytical scientists with the skills andknowledge needed to take full advantage of this new separationtechnology.

High Performance Liquid Chromatography

Author : Ivan Lucero
ISBN : 1536135437
Genre :
File Size : 25. 39 MB
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Ultra Performance Liquid Chromatography Mass Spectrometry

Author : Mu Naushad
ISBN : 9781466591554
Genre : Science
File Size : 82. 46 MB
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Due to its high sensitivity and selectivity, liquid chromatography–mass spectrometry (LC–MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.

Liquid Chromatography

Author : Salvatore Fanali
ISBN : 9780128093443
Genre : Science
File Size : 67. 4 MB
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Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Uhplc In Life Sciences

Author : Davy Guillarme
ISBN : 9781849733885
Genre : Science
File Size : 81. 4 MB
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Since its commercial introduction in 2004, UHPLC (Ultra-High Performance Liquid Chromatography) has begun to replace conventional HPLC in academia and industry and interest in this technique continues to grow. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than 1500 papers have been written on this strongly-evolving topic to date. This book provides a solid background on how to work with UHPLC and its application to the life sciences. The first part of the book covers the basics of this approach and the specifics of a UHPLC system, providing the reader with a solid background to working properly with such a system. The second part examines the application of UHPLC to the life sciences, with a focus on drug analysis strategies. UHPLC-MS, a key technique in pharmaceutical and toxicological analyses, is also examined in detail. The editors (Davy Guillarme and Jean-Luc Veuthey) were some of the earliest adopters of UHPLC and have published and lectured extensively on this topic. Between them they have brought together an excellent team of contributors from Europe and the United States, presenting a wealth of expertise and knowledge. This book is an essential handbook for anyone wishing to adopt an UHPLC system in either an academic or industrial setting and will benefit postgraduate students and experienced workers alike.

Mass Spectrometry Handbook

Author : Mike S. Lee
ISBN : 9781118180723
Genre : Science
File Size : 90. 88 MB
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Due to its enormous sensitivity and ease of use, mass spectrometry has grown into the analytical tool of choice in most industries and areas of research. This unique reference provides an extensive library of methods used in mass spectrometry, covering applications of mass spectrometry in fields as diverse as drug discovery, environmental science, forensic science, clinical analysis, polymers, oil composition, doping, cellular research, semiconductor, ceramics, metals and alloys, and homeland security. The book provides the reader with a protocol for the technique described (including sampling methods) and explains why to use a particular method and not others. Essential for MS specialists working in industrial, environmental, and clinical fields.

Validation Of A Reversed Phase Ultra High Performance Liquid Chromatographic Method With Photodiode Array Detection For The Determination Of Voriconazole In Human Serum And Its Application To Therapeutic Drug Monitoring

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ISBN : OCLC:1053710321
Genre :
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Abstract : Background: Voriconazole is a broad-spectrum triazole antifungal agent. It is widely used in the treatment of invasive fungal infections in immunocompromised patients. Because the pharmacokinetics of voriconazole demonstrates considerable variability, monitoring its serum levels plays an important role in optimizing therapies against many clinically relevant fungal pathogens. The aim of this study was to validate a simple and rapid U-HPLC-PDA method with minimal sample preparation for routine therapeutic drug monitoring (TDM) of voriconazole. Methods: After protein precipitation with the internal standard solution (posaconazole 5.0 mg/L in acetonitrile), chromatographic separation was performed in 4 minutes using water and acetonitrile as mobile phases and an Acquity UPLC BEH HSS C18 column (2.1 × 100 mm, 1.7 μm). The temperature was set at 45°C and the flow rate was 0.4 mL/min. Photodiode array detection at 256 nm was used as detection system. The method was validated according international guidelines for linearity, accuracy, precision, selectivity, lower limit of quantitation, carry over, and stability under different conditions. Results: All performance parameters were within acceptance criteria, demonstrating that the validated method is fit for purpose. After assay validation, 115 serum samples collected from 41 patients were analyzed to report the experience of the laboratory in TDM of voriconazole. Results showed a large variability in voriconazole trough levels, suggesting that this drug should be frequently measured in patients under treatment to enhance therapies efficacy and improve safety. Conclusions: In this study, a reproducible U-HPLC-PDA assay with a short analysis time, requiring only a small amount of serum, good accuracy and reproducibility was validated, which is suitable for routine TDM of voriconazole in serum.

Handbook Of Chemical And Biological Plant Analytical Methods 3 Volume Set

Author : Shilin Chen
ISBN : 9781118874981
Genre : Medical
File Size : 62. 74 MB
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Plants and plant-derived compounds and drugs are becoming moreand more popular with increasing numbers of scientists researchingplant analysis. The quality control of herbal drugs is alsobecoming essential to avoid severe health problems, and in thefuture many more new drugs will be developed from plantsources. This three-volume Handbook, featuring 47 detailed review articles,is unique as it deals with chemical and biological methodologiesfor plant analysis. It presents the most important and mostaccurate methods which are available for plant analysis. This comprehensive work is divided into six sections asfollows: Sample preparation and identification – discussingplant selection and collection, followed by extraction and samplepreparation methodologies. Extraction and sample preparation methodologies Instrumentation for chemical analysis - severalinstrumentations for chemical plant analysis are presented with anemphasis on hyphenated techniques, e.g. the coupling between HPLCand mass spectroscometry, and HPLC with NMR. Strategies for selective classes of compounds –coverage of the most interesting classes of compounds such aspolysaccharides, saponins, cardiotonic glycosides, alkaloids,terpenoids, lipids, volatile compounds and polyphenols (flavonoids, xanthones, coumarins, naphthoquinones, anthraquinones,proanthocyanidins, etc.). Biological Analysis - includes phenotyping, DNA barcodingtechniques, transcriptome analysis , microarray, metabolomics andproteomics. Drugs from Plants – covers the screening of plantextracts and strategies for the quick discovery of novelbioactive natural products. Safety assessment of herbal drugs ishighly dependent on outstanding chromatographic and spectroscopicmethods which are also featured here. This Handbook introduces to scientists involved in plant studiesthe current knowledge of methodologies in various fields ofchemically- and biochemically-related topics in plantresearch. The content from this Handbook will publish online within theEncyclopedia of Analytical Chemistry via Wiley Online Library: ahref="http://www.wileyonlinelibrary.com/ref/eac"http://www.wileyonlinelibrary.com/ref/eac/a Benefit from the introductory offer, valid until 30 November2014! Introductory price: £425.00 / $695.00 /€550.00 List price thereafter: £495.00 / $795.00 / €640.00

Microbiological Applications Of High Performance Liquid Chromatography

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ISBN : UCAL:B5039437
Genre :
File Size : 53. 40 MB
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High Performance Liquid Chromatography In Phytochemical Analysis

Author : Monika Waksmundzka-Hajnos
ISBN : 1420092618
Genre : Science
File Size : 31. 36 MB
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The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field. The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products. Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

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