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Dna Cloning And Assembly

Author : Sunil Chandran
ISBN : 1071609076
Genre : Medical
File Size : 47. 90 MB
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This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Human Genome News

Author :
ISBN : STANFORD:36105214597150
Genre : Human genome
File Size : 67. 28 MB
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Energy Research Abstracts

Author :
ISBN : OSU:32435032775199
Genre : Power resources
File Size : 68. 72 MB
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Mapping Our Genes

Author : United States Government Printing Office
ISBN : UCR:31210010367827
Genre : Biotechnology
File Size : 48. 55 MB
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Molecular Cloning

Author : Joseph Sambrook
ISBN : 0879695765
Genre : Science
File Size : 25. 23 MB
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The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Animal Biotechnology

Author : Ashish S. Verma
ISBN : 9780128117101
Genre : Medical
File Size : 26. 88 MB
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Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare. This updated edition covers vital fundamentals, including animal cell cultures, genome sequencing analysis, epigenetics and animal models, gene expression, and ethics and safety concerns, along with in-depth examples of implications for human health and prospects for the future. New chapters cover animal biotechnology as applied to various disease types and research areas, including in vitro fertilization, human embryonic stem cell research, biosensors, enteric diseases, biopharming, organ transplantation, tuberculosis, neurodegenerative disorders, and more. Highlights the latest biomedical applications of genetically modified and cloned animals, with a focus on cancer and infectious diseases Offers first-hand accounts of the use of biotechnology tools, including molecular markers, stem cells, animal cultures, tissue engineering, ADME and CAM Assay Includes case studies that illustrate safety assessment issues, ethical considerations, and intellectual property rights associated with the translation of animal biotechnology studies

Emery S Elements Of Medical Genetics E Book

Author : Peter D Turnpenny
ISBN : 9780702079672
Genre :
File Size : 76. 75 MB
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Long recognized as a leading textbook in this fast-moving field, Emery's Elements of Medical Genetics and Genomics offers current, complete information with a strong basis in practical clinical genetics and genomics for medical school and beyond. The 16th Edition of this award-winning text has been thoroughly updated throughout and includes case-based and multiple-choice questions, end-of-chapter summaries, an extensive glossary, and convenient online access, making it an ideal choice for all medical undergraduates as well as postgraduates seeking to improve their understanding and knowledge. Includes new case-based studies with questions and answers throughout, in addition to multiple-choice self-assessment questions for study and review. Covers key topics such as pharmacogenetics, personalized medicine, prenatal testing, reproductive genetics, and ethical and legal issues in medical genetics. Divides the text into three easy-to-use sections: The Scientific Basis of Human Genetics, Genetics in Medicine and Genomic Medicine, and Clinical Genetics, Counseling and Ethics. Features full-color illustrations and other images that help readers visualize the appearance of genetic disorders and assist with the understanding of complex genetic structures. Contains learning features such as summary boxes, an extensive glossary of terms, online hyperlinks to important genetics websites and clinical databases, and more. Presents the extensive knowledge and experience of distinguished editors Peter D. Turnpenny and Sian Ellard, as well as new editor Ruth Cleaver.

Proceedings Of The National Academy Of Sciences Of The United States Of America

Author : National Academy of Sciences (U.S.)
ISBN : UCSD:31822018108118
Genre : Science
File Size : 66. 18 MB
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Author : Department of Biomolecular Sciences T a Brown
ISBN : UOM:39015048769445
Genre : Science
File Size : 36. 97 MB
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Responding to the immense changes due to recent development in research, Genomes is the first in a generation of molecular genetics books which combine standard molecular biology with more contemporary genomics. This book focuses on genome organization, expression, replication, and evolution, and includes a description of applications for molecular ecology and anthropology, reflecting the impact of genome biology on other fields of study.


Author : Thomas Lengauer
ISBN : 3527299882
Genre : Science
File Size : 54. 16 MB
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V.1: Basic technologies: From Genomes to drugs with bioinformatics (Thomas Lengauer); Sequence analysis (Martin Vingron); Structure, properties and computer identification of eukaryotic genes (Victor Solovyev). Analyzing regulatory regions in genomes (Thomas Werner). Homology modeling in biology and medicine (Roland L. Dunbrack, Jr.). Protein structure prediction (Ralf Zimmer and Thomas Lengauer). Protein-ligand docking in drug design (Matthias Rarey). Modellimg protein-protein and protein-DNA docking (Michael J. E. Sternberg and Gidon Moont). v.2: Applications: Integrating and accessing molecular biology resources (David P. Hansen and Thure Etzold). Bioinformatics support of genome sequencing projects (Xiaoqiu Huang). Analysis of sequence variations (Christopher S. Carlson, Mark J. Rieder and Deborah A. Nickerson). Proteome analysis (Pierre-Alain Binz, Elisabeth Gasteiger, Jean-Charles Sanchez, Amos Bairoch, Denis F. Hochstrasser, Ron D. Appel). Target finding in genomes and proteomes (Stephanie Fuhrman Liang, Xiling Wen and Roland Somogyi). Screening of drug databases (Martin Stahl, Matthias Rarey and Gerhard Klebe). Future trends (Thomas Lengauer).

Construction And Characterization Of The Bacteriophage Lambda Charon Vectors For Dna Cloning

Author : Bill Gary Williams
ISBN : WISC:89010839728
Genre : Molecular cloning
File Size : 58. 52 MB
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Cloning And Characterization Of Ddrim A Drosophila Gene Similar To Human Down Regulated In Metastasis

Author : Peter J. Merriman
ISBN : WISC:89081079873
Genre :
File Size : 77. 28 MB
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Author : Darbeshwar Roy
ISBN : STANFORD:36105215490751
Genre : Technology & Engineering
File Size : 79. 37 MB
Format : PDF
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Biotechnology provides description of structure and function of the genetic material and the genome organization and discusses in detail genetic code, structural code, regulatory code, RNA world, RNA editing and DNA imprinting. Gene, its structure and expression, gene cloning, in vitro mutagenesis, gene tagging, gene silencing, DNA synthesis and sequencing technology as well as genome sequencing and assembly are also discussed. Molecular marker technology and its applications, structural genomics technologies including methods of analysis of SNPs and haplotypes, functional genomics technologies for studying gene function and interactions at individual gene level and at genome-wide scale and proteomics technologies are fully described. Methods of construction of pathways and networks, and protein synthesis and sequencing technology are included.

Dna Cloning

Author : David M. Glover
ISBN : UOM:39015020767839
Genre : Cloning
File Size : 70. 61 MB
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Spplication of plasmids containing promoters specific for phage-encoded RNA polymerases; Chice and use of cosmid vectors; Genetic approaches to the cloning, modification and characterization of cosmid clones and clone libraries; The purification on eucaryotic polypeptides expressed in Escherichia coli; Production and prufication of polyclonal antibodies to the foreign segment of B-galactosidase fusion proteins; Production of monoclonal antibodies against fusion proteins produced in Escherichia coli; Expression and secretion of foreign genes in yeast; The use of vectors based of gene amplification for the expression of cloned genes in mammalian cells; Retroviral vectors; The production of transgenic mice by the microinjection of cloned DNA into fertilized one-cell eggs.

Methods Of Dna And Rna Sequencing

Author : Sherman M. Weissman
ISBN : UOM:39015006020237
Genre : DNA
File Size : 53. 84 MB
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Provides a comprehensive and scholarly review of the various approaches used for determination of DNA and RNA sequencing, storage, manipulation, and interpretation of primary sequence data in a manner that will explain the logic responsible for both the successes and shortcomings of the current methods.


Author :
ISBN : UOM:39015010072992
Genre : DNA
File Size : 80. 6 MB
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Plant Systems Biology

Author : Dmitry A. Belostotsky
ISBN : UOM:39015075683246
Genre : Science
File Size : 41. 47 MB
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In this authoritative guide, expert investigators provide cutting-edge chapters dealing with modern plant systems biology approaches. This work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Courses And Degrees

Author : Stanford University
ISBN : STANFORD:36105016092046
Genre :
File Size : 52. 52 MB
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Encyclopedia Of Genetics Genomics Proteomics And Bioinformatics 8 Volume Set

Author : Michael J. Dunn
ISBN : 0470849746
Genre : Science
File Size : 23. 30 MB
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Available in print and online, this unique reference brings together all four fields of genetics, genomics, proteomics, and bioinformatics to meet your dynamic research requirements. It brings together the latest concepts in these vibrant areas and ensures a truly multidisciplinary approach. Topics include genetic variation and evolution, epigenetics, the human genome, expression profiling, proteome families, structural proteomics, gene finding/gene structure, protein function and annotation, and more. The work incorporates a vast amount of topical information, profiles cutting-edge techniques, and presents the very latest findings from an international team of over five hundred contributors. With articles for both students and more experienced scientists, this is a key reference source for everyone. Contains more than 450 articles covering all aspects of genomics, proteomics, bioinformatics and related technologies Includes a glossary containing over 550 clear and concise definitions "I am pleased to recommend it heartily as a essential reference tool…should remain the definitive work…for many years to come." THE CHEMICAL EDUCATOR “Jorde…and co-editors have done a remarkable job in coordinating this information, distilling it into a package that is both easy to navigate and over-flowing in discovery." ELECTRIC REVIEW

Methods In Molecular Genetics

Author :
ISBN : IND:30000121314573
Genre : Biochemical genetics
File Size : 31. 95 MB
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