enzyme-mediated-immunoassay

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Enzyme Mediated Immunoassay

Author : H.M. Lenhoff
ISBN : 9781468450125
Genre : Science
File Size : 74. 93 MB
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T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Enzyme Immunoassays

Author : S.S. Deshpande
ISBN : 9781461311690
Genre : Science
File Size : 35. 29 MB
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This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Nonisotopic Immunoassay

Author : That T. Ngo
ISBN : 9781468454666
Genre : Science
File Size : 49. 33 MB
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The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.

Electrochemical Sensors In Immunological Analysis

Author : That T. Ngo
ISBN : 9781489919748
Genre : Medical
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The development of radioimmunoassay (RIA) by R.S. Yalow and S.A. Berson in 1959 opens up a new avenue in ultra sensitive analysis of trace substances in complex biological systems. In recognition of the enormous contributions of RIA to basic research in biology and to routine clinical tests in laboratory medicine, R.S. Yalow, the co-developer of RIA, was awarded, in 1977, the Nobel Prize for Medicine and Physiology. The basic principle of RIA is elegantly simple. It is based on a specific, competitive binding reaction between the analyte and the radio-labeled analog of the analyte for the specific antibody raised to the analyte. The combination of high specificity and affinity of an antibody molecule makes it a very versatile analytical reagent capable of reacting specifically with analytes at a very low concentration in a complex solution such as serum. The sensitivity of RIA is provided by using a radioactive tracer.

Assessment Of Antibody Mediated Pulmonary Immunity In Rats With Enzyme Immunoassay Technology

Author : Charles D. Gradle
ISBN : OCLC:15260181
Genre :
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The Handbook Of Neuropsychiatric Biomarkers Endophenotypes And Genes

Author : Michael S. Ritsner
ISBN : 9789048122981
Genre : Medical
File Size : 79. 13 MB
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Neuropsychiatric disorders such as schizophrenia, mood disorders, Alzheimer’s disease, epilepsy, alcoholism, substance abuse and others are one of the most debilitating illnesses worldwide characterizing by the complexity of the causes, and lacking the laboratory tests that may promote diagnostic and prognostic procedures. Recent advances in neuroscience, genomic, genetic, proteomic and metabolomic knowledge and technologies have opened the way to searching biomarkers and endophenotypes, which may offer powerful and exciting opportunity to understand the etiology and the underlying pathophysiological mechanisms of neuropsychiatric disorders. The challenge now is to translate these advances into meaningful diagnostic and therapeutic advances. This book offers a broad synthesis of the current knowledge about diverse topics of the biomarker and endophenotype strategies in neuropsychiatry. The book is organized into four interconnected volumes: “Neuropsychological Endophenotypes and Biomarkers” (with overview of methodological issues of the biomarker and endophenotype approaches in neuropsychiatry and some technological advances), “Neuroanatomical and Neuroimaging Endophenotypes and Biomarkers”, “Metabolic and Peripheral Biomarkers” and “Molecular Genetic and Genomic Markers”. The contributors are internationally and nationally recognized researchers and experts from 16 countries. This four-volume handbook is intended for a broad spectrum of readers including neuroscientists, psychiatrists, neurologists, endocrinologists, pharmacologists, clinical psychologists, general practitioners, geriatricians, health care providers in the field of neurology and mental health interested in trends that have crystallized in the last decade, and trends that can be expected to further evolve in the coming years. It is hoped that this book will also be a useful resource for the teaching of psychiatry, neurology, psychology and mental health.

Immunoassay

Author : Eleftherios P. Diamandis
ISBN : 0080534503
Genre : Medical
File Size : 51. 10 MB
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Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Presents up-to-date information Provides extensive cross-referencing Covers theory and practice in full detail Written by leading authorities

Immobilized Enzymes In Medicine

Author : Vladimir P. Torchilin
ISBN : 9783642758218
Genre : Science
File Size : 87. 21 MB
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The application of immobilized enzymes in medicine is the main objective of this book. The author reviews natural and synthetic carriers for enzyme immobilization, chemistry of enzyme binding, and in-vitro and in-vivo properties of immobilized enzymes. Four chapters are dedicated to clinical use of immobilized enzymes.

Microfluidic Techniques

Author : Shelley D. Minteer
ISBN : 9781592599974
Genre : Science
File Size : 74. 99 MB
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Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Molecular Biomethods Handbook

Author : Ralph Rapley
ISBN : 9781592596423
Genre : Science
File Size : 80. 17 MB
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In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

Handbook Of Food Analysis

Author : Leo M.L. Nollet
ISBN : 9781482276466
Genre : Technology & Engineering
File Size : 75. 48 MB
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Thoroughly updated to accommodate recent research and state-of-the-art technologies impacting the field, Volume 2: Residues and Other Food Component Analysis of this celebrated 3 volume reference compiles modern methods for the detection of residues in foods from pesticides, herbicides, antibacterials, food packaging, and other sources. Volume 2 ev

Handbook Of Food Analysis Residues And Other Food Component Analysis

Author : Leo M. L. Nollet
ISBN : 0824750373
Genre : Technology & Engineering
File Size : 82. 55 MB
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Thoroughly updated to accommodate recent research and state-of-the-art technologies impacting the field, Volume 2: Residues and Other Food Component Analysis of this celebrated 3 volume reference compiles modern methods for the detection of residues in foods from pesticides, herbicides, antibacterials, food packaging, and other sources. Volume 2 evaluates methods for: establishing the presence of mycotoxins and phycotoxins identifying growth promoters and residual antibacterials tracking residues left by fungicides and herbicides discerning carbamate and urea pesticide residues confirming residual amounts of organochlorine and organophosphate pesticides detecting dioxin, polychlorobiphenyl (PCB), and dioxin-like PCB residues ascertaining n-nitroso compounds and polycyclic aromatic hydrocarbons tracing metal contaminants in foodstuffs

Clinical Chemistry

Author : E. Howard Taylor
ISBN : UOM:39015018309941
Genre : Medical
File Size : 42. 90 MB
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An introduction to the theory and practice of clinical chemistry, integrating basic analytical concepts with the diagnosis of disease. Presents a balanced approach, emphasizing analysis and interpretation and covering electrolytes and acid-base, renal function, proteins, enzyme analysis, hormone immunoassays, coagulation, therapeutic drug monitoring, and laboratory evaluation of nutritional status. Also discusses sample integrity, offering steps to evaluate quality control and quality assurance.

Hazard Assessment Of Chemicals

Author : J. Saxena
ISBN : 1560322713
Genre : Science
File Size : 42. 81 MB
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The majority of chapters in this volume are devoted to various aspects of health risk assessment the evaluation of health hazard associated with human risk exposure to environmental chemicals. Among topics discussed

Modern Medical Toxicology

Author : Pillay
ISBN : 9789350259658
Genre :
File Size : 59. 70 MB
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Russian Chemical Reviews

Author :
ISBN : UOM:39015026414451
Genre : Chemistry
File Size : 55. 63 MB
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Drugs For Heart Disease

Author : John Hamer
ISBN : 9781489932945
Genre : Medical
File Size : 48. 8 MB
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This book is the outcome of a cardiologist switching to clinical pharma cology in mid-career and may be seen as representing the interface between the two disciplines. In this second edition I have not tried to be encyclopaedic, but have asked the contributors to give a brief account of current practice, so that it represents the present state of cardiac thera peutics. Although some contributors are from Bart's, I have tried to spread my net widely and produce a general view from the English speaking world. I hope this will be enough to draw the teeth of my colleagues who will react at once to say that 'this is not what we do at Bart's. ' Some chapters, mostly the early ones, are drug orientated and describe the use and properties of individual groups of drugs. Other later chapters are disease orientated and describe the use of various groups of drugs in different conditions, such as angina or hypertension. This necessarily leads to some overlap, but I have not tried to produce a uniformity of view, but have been content with Chairman Mao 'to let a hundred flowers bloom'. I am grateful to my cardiological colleague, Professor A. John Camm for his help and advice on current cardiological practice. To improve the flow of the text I have limited the references to key publications, rather than trying to quote all the papers published on each topic.

Clinical And Translational Science

Author : David Robertson
ISBN : 0080920195
Genre : Medical
File Size : 24. 79 MB
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Clinical or translational science is the field of study devoted to investigating human health and disease, interventions and outcomes for the purposes of developing new treatment approaches, devices, and modalities to improve health. New molecular tools and diagnostic technologies based on clinical and translational research have lead to a better understanding of human disease and the application of new therapeutics for enhanced health. Clinical and Translational Science is designed as the most authoritative and modern resource for the broad range of investigators in various medical specialties taking on the challenge of clinical research. Prepared with an international perspective, this resource begins with experimental design and investigative tools to set the scene for readers. It then moves on to human genetics and pharmacology with a focus on statistics, epidemiology, genomic information, drug discovery and development, and clinical trials. Finally, it turns to legal, social, and ethical issues of clinical research concluding with a discussion of future prospects to provide readers with a comprehensive view of the this developing area of science. Clinical research is one of the fastest growing fields in private practice and academic medicine with practical biological, physiological, cellular, and therapeutic applications Contributions from international leaders provide insight into background and future understanding for clinical and translational science Provides the structure for complete instruction and guidance on the subject from fundamental principles, approaches and infrastructure to human genetics, human pharmacology, research in special populations, the societal context of human research, and the future of human research

Immunodiagnosis Of Cancer

Author : M.d d.
ISBN : 9781351439244
Genre : Science
File Size : 60. 74 MB
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Updated to reflect changes in the field since publication of the first edition in 1979. Provides a detailed review of the methodology available for assessing the diagnosis and prognosis of cancer patients including data on the application of tumor marker assays and other immunodiagnostic procedures

Trace Analysis

Author : Peter Bedson
ISBN : 9781847559401
Genre : Science
File Size : 52. 55 MB
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Trace Analysis is a highly practical book which deals with the science rather than the paperwork of quality assurance systems. Produced as part of the UK Valid Analytical Measurement (VAM) initiative, it provides the analyst with a systematic approach across the broad spectrum of trace analysis, offering practical advice and guidance on methodology and techniques. The book is structured to take the analyst step-by-step through the stages of any trace analysis. The approach is general, being broken down only into types of analyte. Additional chapters explain the application of groups of techniques to each analyte type. Each section contains references to published material which will allow the analyst to obtain further information on specific topics. Throughout the book, the analyst is reminded of pitfalls which lead to unreliable results. This new book therefore offers invaluable advice to analysts in all areas and at all levels, providing practical 'expert' advice on methodology. It will prove indispensable as a single, comprehensive bench guide for analysts in university, college and industrial laboratories.

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