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Molecular Biology Techniques

Author : Sue Carson
ISBN : 9780123855442
Genre : Medical
File Size : 47. 66 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Biology Techniques

Author : Sue Carson
ISBN : 9780128157756
Genre : Science
File Size : 89. 81 MB
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Techniques For Molecular Biology

Author : D Tagu
ISBN : 1578083613
Genre : Science
File Size : 22. 99 MB
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With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.

Morphology Methods

Author : Ricardo V. Lloyd
ISBN : 9781592591909
Genre : Medical
File Size : 59. 40 MB
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The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Molecular Biology Techniques

Author : Walt Ream
ISBN : 9780080536828
Genre : Science
File Size : 63. 94 MB
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Principles And Techniques Of Biochemistry And Molecular Biology

Author : Keith Wilson
ISBN : 9781107393493
Genre : Science
File Size : 48. 10 MB
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This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Techniques In Molecular Biology

Author : J.M. Walker
ISBN : 9789401165631
Genre : Science
File Size : 29. 96 MB
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The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Wood Formation In Trees

Author : Nigel J Chaffey
ISBN : 0203166442
Genre : Science
File Size : 27. 19 MB
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Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of

Exam Prep For Molecular Biology Techniques

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Basic Techniques In Molecular Biology

Author : Stefan Surzycki
ISBN : 9783642569685
Genre : Science
File Size : 77. 36 MB
Format : PDF
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Laboratory Techniques In Biochemistry And Molecular Biology Techniques Of Lipidology

Author : Jorgen Clausen
ISBN : 0720442001
Genre : Biochemistry
File Size : 64. 27 MB
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Basic Techniques In Biochemistry And Molecular Biology

Author : R. K. Sharma
ISBN : 9788190746267
Genre : Biochemistry
File Size : 73. 2 MB
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Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.

Molecular Biology Techniques

Author : Source Wikipedia
ISBN : 1230545654
Genre :
File Size : 54. 60 MB
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 92. Chapters: Polymerase chain reaction, Plasmid, Southern blot, Northern blot, Gene knockout, Western blot, Cell culture, DNA sequencing, DNA microarray, Immunoprecipitation, Transmission electron microscopy DNA sequencing, Methods to investigate protein-protein interactions, Exome sequencing, ChIP-on-chip, Forster resonance energy transfer, Nucleic acid structure determination, Chromatin immunoprecipitation, Eastern blotting, ChIA-PET, COLD-PCR, Paired-end Tags, Combined bisulfite restriction analysis, Primer dimer, Kodecyte, DNA footprinting, Suspension array technology, PBR322, Allele-specific oligonucleotide, Chip-Sequencing, Streptamer, Oligomer restriction, Reverse transfection, Strep-tag, Selection and amplification binding assay, Knockout moss, Native PAGE, Isoelectric focusing, Helicase-dependent amplification, Subcloning, Plant transformation vector, Chemically defined medium, Magnet-assisted transfection, Branched DNA assay, TA cloning, Nuclease protection assay, Bio-Layer Interferometry, Southwestern blot, Synchronous culture, MassTag-PCR, Far-Eastern blotting, Ribosomal Intergenic Spacer analysis, Ligase chain reaction, Isopeptag, Far-western blotting, Gene knockin, Baby hamster kidney cell, Univec, VectorDB, Chromogenic in situ hybridization, Alanine scanning, Calcium chloride transformation, Fluorophore assisted carbohydrate electrophoresis, Reverse northern blot, Gel Doc, Racker unit, Promoter bashing, PBLU.

Laboratory Techniques In Biochemistry And Molecular Biology

Author : Roy Hunter Burdon
ISBN : 0444807373
Genre : Biochemistry
File Size : 58. 48 MB
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Analytical Techniques In Biochemistry And Molecular Biology

Author : Rajan Katoch
ISBN : 1441997857
Genre : Science
File Size : 59. 51 MB
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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Algorithms In Computational Molecular Biology

Author : Mourad Elloumi
ISBN : 9781118101988
Genre : Science
File Size : 87. 43 MB
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This book represents the most comprehensive and up-to-date collection of information on the topic of computational molecular biology. Bringing the most recent research into the forefront of discussion, Algorithms in Computational Molecular Biology studies the most important and useful algorithms currently being used in the field, and provides related problems. It also succeeds where other titles have failed, in offering a wide range of information from the introductory fundamentals right up to the latest, most advanced levels of study.

Experiments In Molecular Biology

Author : Zachary F. Burton
ISBN : 0121473708
Genre : Science
File Size : 22. 82 MB
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Experiments in Molecular Biology provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry. This unique laboratory manual is particularly appropriate for courses in molecular cloning, molecular genetics techniques, molecular biology techniques, recombinant DNA techniques, bacterial genetics techniques, and genetic engineering. Included is an especially helpful section to aid new instructors in avoiding potential pitfalls of specific experiments. Key Features * Contains student-tested, easy-to-follow protocols * Presents background information that reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying molecular biochemistry * Includes student-tested, easy-to-follow protocols * Background information reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Advises new instructors on potential pitfalls of specific experiments * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying

Computational Methods In Molecular Biology

Author : S.L. Salzberg
ISBN : 0080860931
Genre : Computers
File Size : 84. 60 MB
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Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Articles On Molecular Biology Techniques Including Northern Blot Polymerase Chain Reaction Plasmid Southern Blot Gene Knockout Western Blot Dn

Author : Hephaestus Books
ISBN : 1243242841
Genre : Social Science
File Size : 49. 75 MB
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Hephaestus Books represents a new publishing paradigm, allowing disparate content sources to be curated into cohesive, relevant, and informative books. To date, this content has been curated from Wikipedia articles and images under Creative Commons licensing, although as Hephaestus Books continues to increase in scope and dimension, more licensed and public domain content is being added. We believe books such as this represent a new and exciting lexicon in the sharing of human knowledge. This particular book is a collaboration focused on Molecular biology techniques.

Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology

Author : Andreas Hofmann
ISBN : 9781107162273
Genre : Medical
File Size : 54. 85 MB
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A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

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