nonisotopic-immunoassay

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Nonisotopic Immunoassay

Author : That T. Ngo
ISBN : 9781468454666
Genre : Science
File Size : 90. 2 MB
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The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.

The Immunoassay Handbook

Author : David Wild
ISBN : 0080445268
Genre : Medical
File Size : 38. 78 MB
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Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.

Advances In Clinical Chemistry

Author :
ISBN : 0080566227
Genre : Science
File Size : 58. 12 MB
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Advances in Clinical Chemistry

Immunochemistry Of Solid Phase Immunoassay

Author : John E. Butler
ISBN : 0849353947
Genre : Science
File Size : 24. 11 MB
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Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

Enzyme Immunoassay

Author : Edward T. Maggio
ISBN : 9781351080293
Genre : Health & Fitness
File Size : 62. 21 MB
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The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.

Immunoassay Automation

Author : Daniel W. Chan
ISBN : 9780323137812
Genre : Medical
File Size : 37. 16 MB
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Immunoassay Automation: A Practical Guide describes automation of immunoassay from the practical viewpoint of the clinical laboratory. General introduction and evaluation sections demonstrate principles and practice. A comprehensive selection of available systems are detailed by experts, with a view towards popularity, technical advances, and operational efficiency. This laboratory guide is essential for practitioners in clinical chemistry laboratories, and will have lasting value in the evolution of automated systems. Focuses on automation of immunoassay for the clinical laboratory Emphasizes principles, method evaluation, and the systems approach Aids system selection by evaluation of technical, clinical, operational, and economical parameters Contains complete descriptions by experts on the latest automated immunoassay systems Based upon the editor's well-received workshops on automated immunoassay

Immunoassay Technology

Author : S. B. Pal
ISBN : 9783110864199
Genre : Science
File Size : 41. 90 MB
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Enzyme Immunoassays

Author : S.S. Deshpande
ISBN : 9781461311690
Genre : Science
File Size : 33. 95 MB
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This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.

Handbook Of Analytical Therapeutic Drug Monitoring And Toxicology 1996

Author : Steven H.Y. Wong
ISBN : 9781351364287
Genre : Medical
File Size : 57. 46 MB
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Adapting modern advances in analytical techniques to daily laboratory practices challenges many toxicologists, clinical laboratories, and pharmaceutical scientists. The Handbook of Analytical Therapeutic Drug Monitoring and Toxicology helps you keep abreast of the innovative changes that can make your laboratory - and the studies undertaken in it - a success. This volume simplifies your search for appropriate techniques, describes recent contributions from leading investigators, and provides valuable evaluations and advice.

Principles Of Perinatal Neonatal Metabolism

Author : Richard M. Cowett
ISBN : 9781461216421
Genre : Medical
File Size : 88. 49 MB
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Here, a stellar international group of contributors examines the various aspects of metabolism in the human adult during pregnancy, in the foetus, and in the new-born. This second edition has been completely updated and now has more than 17 new chapters. Divided into five sections, the book contains discussions of methodologies using molecular biology techniques, expanded coverage of central nervous system metabolism, and an entirely new section on organ-specific metabolism organised according to each organ-system. A must for every physician who cares for the pregnant patient and her child.

Bioaffinity Chromatography

Author : J. Turková
ISBN : 0080858635
Genre : Science
File Size : 69. 46 MB
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Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.

Principles And Practice Of Immunoassay

Author : Christopher P. Price
ISBN : 9781349112340
Genre : Medical
File Size : 81. 80 MB
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Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.

Luminescence Immunoassay And Molecular Applications

Author : Knox Van Dyke
ISBN : 0849358655
Genre : Medical
File Size : 89. 30 MB
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This unique, one-of-a-kind book provides updated information on the various aspects of luminescence immunoassay (LIA). Focusing on this newly emerging technology, this work describes the important findings regarding the chemistry of light and the various forms of luminescent immunoassay. It includes antigen labeling, enzyme labeling, cofactor labeling, enzyme multiplied assay, and multiple labeled immunoassay. This interesting text also discusses calculations, including computer programs available for high throughput analysis. No other compendium provides such a broad overview, as well as details on this fascinating subject. Both novices and experts will find important and useful information packed in this must-have resource on luminescence immunoassay.

Immunoassay Technology Vol 2

Author : S. B. Pal
ISBN : 9783110884692
Genre : Science
File Size : 36. 7 MB
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Topics In Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
ISBN : 9780306470608
Genre : Science
File Size : 49. 4 MB
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Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Immunoassay

Author : Gerard Meurant
ISBN : 9780323137829
Genre : Medical
File Size : 48. 11 MB
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During the last few years, immunoassay has gained tremendous popularity in clinical and research laboratories and has been applied to determine hormone, enzyme, protein, drug, and infectious agents. The aim of this book is to provide clinical laboratory personnel and students with an understanding of the principle of immunoassay and the production of reagents for immunoassay.

Immunoassay

Author : Eleftherios P. Diamandis
ISBN : 0080534503
Genre : Medical
File Size : 30. 74 MB
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Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Presents up-to-date information Provides extensive cross-referencing Covers theory and practice in full detail Written by leading authorities

Inhibin Activin And Follistatin

Author : Toshihiro Aono
ISBN : 9781461218746
Genre : Health & Fitness
File Size : 42. 11 MB
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This volume is the proceedings of the International Conference on Inhibin, Activin: Recent Advances and Future Views held in Tokushima, Japan from November 9-10, 1996. The Internationally recognized faculty present the latest research in the exploration of inhibin, activin and follistatin mechanisms of action.

Enzyme Mediated Immunoassay

Author : H.M. Lenhoff
ISBN : 9781468450125
Genre : Science
File Size : 58. 86 MB
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T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Reviews On Immunoassay Technology

Author : S.B. Pal
ISBN : 9781349103188
Genre : Medical
File Size : 43. 19 MB
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The books in this series comprise review articles and descriptions of methods of immunoassay, which are not reliant on radioisotopes and reflect the growing importance of immunoassay technologies.

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