optical fluorescence microscopy from the spectral to the nano dimension

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Optical Fluorescence Microscopy

Author : Alberto Diaspro
ISBN : 3642151752
Genre : Science
File Size : 87. 85 MB
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In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Fundamentals Of Fluorescence Microscopy

Author : Partha Pratim Mondal
ISBN : 9789400775459
Genre : Science
File Size : 81. 34 MB
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This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Novel Approaches For Single Molecule Activation And Detection

Author : Fabio Benfenati
ISBN : 9783662433676
Genre : Science
File Size : 46. 50 MB
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How can we obtain tools able to process and exchange information at the molecular scale? In order to do this, it is necessary to activate and detect single molecules under controlled conditions. This book focuses on the generation of biologically-inspired molecular devices. These devices are based on the developments of new photonic tools able to activate and stimulate single molecule machines. Additionally, new light sensitive molecules can be selectively activated by photonic tools. These technological innovations will provide a way to control activation of single light-sensitive molecules, allowing the investigation of molecular computation in a biological environment.

Nanotechnology In Catalysis 3 Volumes

Author : Bert Sels
ISBN : 9783527339143
Genre : Science
File Size : 61. 60 MB
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Reflecting the R&D efforts in the field that have resulted in a plethora of novel applications over the past decade, this handbook gives a comprehensive overview of the tangible benefits of nanotechnology in catalysis. By bridging fundamental research and industrial development, it provides a unique perspective on this scientifically and economically important field. While the first three parts are devoted to preparation and characterization of nanocatalysts, the final three provide in-depth insights into their applications in the fine chemicals industry, the energy industry, and for environmental protection, with expert authors reporting on real-life applications that are on the brink of commercialization. Timely reading for catalytic chemists, materials scientists, chemists in industry, and process engineers.

Chemical Imaging Analysis

Author : Freddy Adams
ISBN : 9780444634504
Genre : Science
File Size : 85. 19 MB
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Chemical Imaging Analysis covers the advancements made over the last 50 years in chemical imaging analysis, including different analytical techniques and the ways they were developed and refined to link the composition and structure of manmade and natural materials at the nano/micro scale to the functional behavior at the macroscopic scale. In a development process that started in the early 1960s, a variety of specialized analytical techniques was developed – or adapted from existing techniques – and these techniques have matured into versatile and powerful tools for visualizing structural and compositional heterogeneity. This text explores that journey, providing a general overview of imaging techniques in diverse fields, including mass spectrometry, optical spectrometry including X-rays, electron microscopy, and beam techniques. Provides comprehensive coverage of analytical techniques used in chemical imaging analysis Explores a variety of specialized techniques Provides a general overview of imaging techniques in diverse fields

Energy Materials Coordinating Committe Emacc Fiscal Year 2004 Annual Technical Report

Author :
ISBN : 9781422345597
Genre :
File Size : 69. 21 MB
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Nano Optics And Near Field Optical Microscopy

Author : Anatoly V. Zayats
ISBN : 9781596932845
Genre : Electronic books
File Size : 61. 31 MB
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This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities.

The Handbook Of Nanomedicine

Author : Kewal K. Jain
ISBN : 1603273190
Genre : Science
File Size : 90. 2 MB
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This handbook covers the broad scope of nanomedicine. Starting with the basics, the subject is developed to potential clinical applications, many of which are still at an experimental stage. The book features extensive coverage of nanodiagnostics and nanopharmaceuticals, which are two important components of nanomedicine. Written by a physician-scientist author who blends his clinical experience and scientific expertise in new technologies, this book provides a definitive account of nanomedicine. It offers more up-to-date and comprehensive coverage of nanomedicine than any other comparable work.

Atomic Force Microscopy Scanning Nearfield Optical Microscopy And Nanoscratching

Author : Gerd Kaupp
ISBN : 9783540284727
Genre : Technology & Engineering
File Size : 75. 90 MB
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Making a clear distinction is made between nano- and micro-mechanical testing for physical reasons, this monograph describes the basics and applications of the supermicroscopies AFM and SNOM, and of the nanomechanical testing on rough and technical natural surfaces in the submicron range down to a lateral resolution of a few nm. New or improved instrumentation, new physical laws and unforeseen new applications in all branches of natural sciences (around physics, chemistry, mineralogy, materials science, biology and medicine) and nanotechnology are covered as well as the sources for pitfalls and errors. It outlines the handling of natural and technical samples in relation to those of flat standard samples and emphasizes new special features. Pitfalls and sources of errors are clearly demonstrated as well as their efficient remedy when going from molecularly flat to rough surfaces. The academic or industrial scientist learns how to apply the principles for tackling their scientific or manufacturing tasks that include roughness far away from standard samples.

Optics Letters

Author :
ISBN : CHI:82280891
Genre : Optics
File Size : 48. 44 MB
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